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Literature summary for 3.5.5.7 extracted from
- A novel nitrilase from Rhodobacter sphaeroides LHS-305: cloning, heterologous expression and biochemical characterization (2014), World J. Microbiol. Biotechnol., 30, 245-252.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli Rosetta (DE3) cells | Cereibacter sphaeroides |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 1% residual activity at 5 mM | Cereibacter sphaeroides |  |
| Ca2+ | 60% residual activity at 5 mM | Cereibacter sphaeroides | |
| Co2+ | 75% residual activity at 5 mM | Cereibacter sphaeroides | |
| Cu2+ | 43% residual activity at 5 mM | Cereibacter sphaeroides | |
| EDTA | 63% residual activity at 5 mM | Cereibacter sphaeroides | |
| Fe2+ | 44% residual activity at 5 mM | Cereibacter sphaeroides | |
| Mg2+ | 93% residual activity at 5 mM | Cereibacter sphaeroides | |
| Ni2+ | 87% residual activity at 5 mM | Cereibacter sphaeroides | |
| Zn2+ | 19% residual activity at 5 mM | Cereibacter sphaeroides | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 73.1 | - |
3-Cyanopyridine | at pH 7.0 and 30°C | Cereibacter sphaeroides | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
14 * 40000, SDS-PAGE | Cereibacter sphaeroides |
| 560000 | - |
gel filtration | Cereibacter sphaeroides |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cereibacter sphaeroides | G5DDB2 | - |
- |
| Cereibacter sphaeroides LHS-305 | G5DDB2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography, and gel filtration | Cereibacter sphaeroides |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,4-dicyanobutane + H2O | 114.3% activity compared to 3-cyanopyridine | Cereibacter sphaeroides | ? | - |
? | |
| 1,8-dicyanooctane + H2O | 114.3% activity compared to 3-cyanopyridine | Cereibacter sphaeroides | ? | - |
? | |
| 2-methylglutaronitrile + 4 H2O | 23.3% activity compared to 3-cyanopyridine | Cereibacter sphaeroides | 2-methylglutarate + 2 NH3 | - |
? | |
| 3-cyanopyridine + 2 H2O | 100% activity | Cereibacter sphaeroides | pyridine 3-carboxylic acid + NH3 | - |
? | |
| 3-cyanopyridine + 2 H2O | 100% activity | Cereibacter sphaeroides LHS-305 | pyridine 3-carboxylic acid + NH3 | - |
? | |
| 3-phenylpropionitrile + H2O | 147.3% activity compared to 3-cyanopyridine | Cereibacter sphaeroides | 3-phenylpropanoate + NH3 | - |
? | |
| 4-chlorobutyronitrile + H2O | 135% activity compared to 3-cyanopyridine | Cereibacter sphaeroides | 4-chlorobutyrate + NH3 | - |
? | |
| 4-chlorobutyronitrile + H2O | 135% activity compared to 3-cyanopyridine | Cereibacter sphaeroides LHS-305 | 4-chlorobutyrate + NH3 | - |
? | |
| acrylonitrile + H2O | 25.9% activity compared to 3-cyanopyridine | Cereibacter sphaeroides | acrylate + NH3 | - |
? | |
| benzonitrile + 2 H2O | 55.4% activity compared to 3-cyanopyridine | Cereibacter sphaeroides | benzoate + NH3 | - |
? | |
| benzonitrile + 2 H2O | 55.4% activity compared to 3-cyanopyridine | Cereibacter sphaeroides LHS-305 | benzoate + NH3 | - |
? | |
| dodecanenitrile + H2O | 13.5% activity compared to 3-cyanopyridine | Cereibacter sphaeroides | dodecanoate + NH3 | - |
? | |
| fumaronitrile + H2O | 209.8 activity compared to 3-cyanopyridine | Cereibacter sphaeroides | fumarate + NH3 | - |
? | |
| additional information | no activity with iminodiacetonitrile, glycolonitrile, 3-hydroxyglutaronitrile, phenylacetonitrile, 1,2-phenylenediacetonitrile, mandelonitrile, and indole-3-acetonitrile | Cereibacter sphaeroides | ? | - |
? | |
| additional information | no activity with iminodiacetonitrile, glycolonitrile, 3-hydroxyglutaronitrile, phenylacetonitrile, 1,2-phenylenediacetonitrile, mandelonitrile, and indole-3-acetonitrile | Cereibacter sphaeroides LHS-305 | ? | - |
? | |
| pentanenitrile + H2O | 62% activity compared to 3-cyanopyridine | Cereibacter sphaeroides | pentanoate + NH3 | - |
? | |
| succinonitrile + H2O | 66.3% activity compared to 3-cyanopyridine | Cereibacter sphaeroides | succinate + NH3 | - |
? | |
| succinonitrile + H2O | 66.3% activity compared to 3-cyanopyridine | Cereibacter sphaeroides LHS-305 | succinate + NH3 | - |
? | |
| thiophene-3-carbonitrile + H2O | 51.2% activity compared to 3-cyanopyridine | Cereibacter sphaeroides | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetradecamer | 14 * 40000, SDS-PAGE | Cereibacter sphaeroides |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Cereibacter sphaeroides |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 50 | about 55% activity at 30°C, about 80% activity at 35°C, 100% activity at 40°C, about 70% activity at 45°C, and about 30% activity at 50°C | Cereibacter sphaeroides |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Cereibacter sphaeroides |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | about 60% activity at pH 6.0 and 80% activity at pH 8.0 in 10 mM sodium phosphate | Cereibacter sphaeroides |
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