Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas fluorescens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 1 mM, 8% residual activity | Pseudomonas fluorescens | |
Cu2+ | 1 mM, no residual activity | Pseudomonas fluorescens | |
Hg2+ | 1 mM, 10% residual activity | Pseudomonas fluorescens | |
N-ethylmaleimide | 0.1 mM, 48% residual activity | Pseudomonas fluorescens | |
pyridoxal 5'-phosphate | 0.1 mM, 30% residual activity | Pseudomonas fluorescens | |
Zn2+ | 1 mM, no residual activity | Pseudomonas fluorescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | 3 | Fumaronitrile | pH 7.0, 30°C | Pseudomonas fluorescens | |
17.9 | - |
Succinonitrile | pH 7.0, 30°C | Pseudomonas fluorescens | |
23.3 | - |
acrylonitrile | pH 7.0, 30°C | Pseudomonas fluorescens | |
23.8 | - |
Benzonitrile | pH 7.0, 30°C | Pseudomonas fluorescens | |
24 | - |
glutaronitrile | pH 7.0, 30°C | Pseudomonas fluorescens | |
25.3 | - |
sebaconitrile | pH 7.0, 30°C | Pseudomonas fluorescens | |
43.9 | - |
Propionitrile | pH 7.0, 30°C | Pseudomonas fluorescens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
4 * 33000, SDS-PAGE | Pseudomonas fluorescens |
138000 | - |
gel filtration | Pseudomonas fluorescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | Q4KCL8 | - |
- |
Pseudomonas fluorescens Pf-5 | Q4KCL8 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.7 | - |
substrate succinonitrile | Pseudomonas fluorescens |
1.9 | - |
substrate fumaronitrile | Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acrylonitrile + H2O | - |
Pseudomonas fluorescens | acrylic acid + NH3 | - |
? | |
acrylonitrile + H2O | - |
Pseudomonas fluorescens Pf-5 | acrylic acid + NH3 | - |
? | |
benzonitrile + 2 H2O | - |
Pseudomonas fluorescens | benzoate + NH3 | - |
? | |
fumaronitrile + H2O | - |
Pseudomonas fluorescens | fumaric acid + NH3 | - |
? | |
fumaronitrile + H2O | - |
Pseudomonas fluorescens Pf-5 | fumaric acid + NH3 | - |
? | |
glutaronitrile + H2O | - |
Pseudomonas fluorescens | glutarate + NH3 | - |
? | |
additional information | enzyme displays a high specificity towards dinitrile substrates | Pseudomonas fluorescens | ? | - |
? | |
additional information | enzyme displays a high specificity towards dinitrile substrates | Pseudomonas fluorescens Pf-5 | ? | - |
? | |
propionitrile + H2O | - |
Pseudomonas fluorescens | propionate + NH3 | - |
? | |
propionitrile + H2O | - |
Pseudomonas fluorescens Pf-5 | propionate + NH3 | - |
? | |
sebaconitrile + H2O | - |
Pseudomonas fluorescens | ? + NH3 | - |
? | |
succinonitrile + H2O | - |
Pseudomonas fluorescens | succinate + NH3 | - |
? | |
succinonitrile + H2O | - |
Pseudomonas fluorescens Pf-5 | succinate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 33000, SDS-PAGE | Pseudomonas fluorescens |
Synonyms | Comment | Organism |
---|---|---|
NitPf5 | - |
Pseudomonas fluorescens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Pseudomonas fluorescens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
16 | - |
50% of initial activity after 80 h | Pseudomonas fluorescens |
30 | - |
50% of initial activity after 30 h | Pseudomonas fluorescens |
45 | - |
50% of initial activity after 300 min | Pseudomonas fluorescens |
55 | - |
50% of initial activity after 180 min | Pseudomonas fluorescens |
65 | - |
50% of initial activity after 45 min | Pseudomonas fluorescens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
Propionitrile | pH 7.0, 30°C | Pseudomonas fluorescens | |
0.22 | - |
Benzonitrile | pH 7.0, 30°C | Pseudomonas fluorescens | |
0.33 | - |
acrylonitrile | pH 7.0, 30°C | Pseudomonas fluorescens | |
3.3 | - |
sebaconitrile | pH 7.0, 30°C | Pseudomonas fluorescens | |
5 | - |
glutaronitrile | pH 7.0, 30°C | Pseudomonas fluorescens | |
8.8 | - |
Succinonitrile | pH 7.0, 30°C | Pseudomonas fluorescens | |
9.2 | - |
Fumaronitrile | pH 7.0, 30°C | Pseudomonas fluorescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Pseudomonas fluorescens |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pseudomonas fluorescens | isoelectric focusing and calculated | - |
5.4 |