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Literature summary for 3.5.5.7 extracted from

  • Kobayashi, M.; Yanak, N.; Nagasawa, T.; Yamada, H.
    Primary structure of an aliphatic nitrile-degrading enzyme, aliphatic nitrilase, from Rhodococcus rhodocrous K22 and expression of its gene and identification of its active site residue (1992), Biochemistry, 31, 9000-9007.
    View publication on PubMed
Search for more literature for 3.5.5.7

Cloned(Commentary)

Cloned (Comment) Organism
overproduction in Escherichia coli Rhodococcus rhodochrous

Protein Variants

Protein Variants Comment Organism
C170A mutant enzymes Cys170Ala and Cys170Ser show no catalytic activity Rhodococcus rhodochrous
C170S mutant enzymes Cys170Ala and Cys170Ser show no catalytic activity Rhodococcus rhodochrous

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42275
-
 x * 42275, calculation from nucleotide sequence Rhodococcus rhodochrous

Organism

Organism UniProt Comment Textmining
Rhodococcus rhodochrous
-
-
-
Rhodococcus rhodochrous K22
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rhodococcus rhodochrous

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acrylonitrile + H2O
-
 Rhodococcus rhodochrous acrylic acid + NH3
-
 ?
acrylonitrile + H2O
-
 Rhodococcus rhodochrous K22 acrylic acid + NH3
-
 ?
crotonitrile + H2O
-
 Rhodococcus rhodochrous crotonic acid + NH3
-
 ?
crotonitrile + H2O
-
 Rhodococcus rhodochrous K22 crotonic acid + NH3
-
 ?
glutaronitrile + H2O
-
 Rhodococcus rhodochrous glutarate + NH3
-
 ?
glutaronitrile + H2O
-
 Rhodococcus rhodochrous K22 glutarate + NH3
-
 ?

Subunits

Subunits Comment Organism
? x * 42275, calculation from nucleotide sequence Rhodococcus rhodochrous