Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM109 cells | Pseudomonas fluorescens |
Protein Variants | Comment | Organism |
---|---|---|
E137A | the mutant demonstrates less than 1% of the wild type activity but is still enzymatically competent to convert mandelonitrile to mandelic acid and mandeloamide | Pseudomonas fluorescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
- |
- |
Pseudomonas fluorescens EBC191 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R,S)-2-phenylpropionitrile + 2 H2O | - |
Pseudomonas fluorescens | (S)-2-phenylpropionic acid + NH3 | ee 66% | ? | |
(R,S)-2-phenylpropionitrile + 2 H2O | - |
Pseudomonas fluorescens EBC191 | (S)-2-phenylpropionic acid + NH3 | ee 66% | ? | |
(R,S)-mandelonitrile + 2 H2O | - |
Pseudomonas fluorescens | (R)-mandelic acid + NH3 | ee 22% | ? | |
(R,S)-mandelonitrile + 2 H2O | - |
Pseudomonas fluorescens EBC191 | (R)-mandelic acid + NH3 | ee 22% | ? |
Synonyms | Comment | Organism |
---|---|---|
Arylacetonitrilase | - |
Pseudomonas fluorescens |
NitA | - |
Pseudomonas fluorescens |