Literature summary for 3.5.5.5 extracted from

Sosedov, O.; Stolz, A.
Improvement of the amides forming capacity of the arylacetonitrilase from Pseudomonas fluorescens EBC191 by site-directed mutagenesis (2015), Appl. Microbiol. Biotechnol., 99, 2623-2635.

Search for more literature for 3.5.5.5

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli JM109 cells	Pseudomonas fluorescens

Protein Variants

Protein Variants	Comment	Organism
E137A	the mutant demonstrates less than 1% of the wild type activity but is still enzymatically competent to convert mandelonitrile to mandelic acid and mandeloamide	Pseudomonas fluorescens

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas fluorescens	-	-	-
Pseudomonas fluorescens EBC191	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(R,S)-2-phenylpropionitrile + 2 H2O	-	Pseudomonas fluorescens	(S)-2-phenylpropionic acid + NH3	ee 66%	?
(R,S)-2-phenylpropionitrile + 2 H2O	-	Pseudomonas fluorescens EBC191	(S)-2-phenylpropionic acid + NH3	ee 66%	?
(R,S)-mandelonitrile + 2 H2O	-	Pseudomonas fluorescens	(R)-mandelic acid + NH3	ee 22%	?
(R,S)-mandelonitrile + 2 H2O	-	Pseudomonas fluorescens EBC191	(R)-mandelic acid + NH3	ee 22%	?

Synonyms

Synonyms	Comment	Organism
Arylacetonitrilase	-	Pseudomonas fluorescens
NitA	-	Pseudomonas fluorescens

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)