Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.5.4 extracted from

  • Yanase, H.; Sakai, T.; Tonomura, K.
    Metabolism of nitriles in microorganisms. Part V. Purification, crystallization and some properties of beta-cyano-L-alanine-degrading enzyme in Pseudomonas sp. 13 (1983), Agric. Biol. Chem., 47, 473-482.
No PubMed abstract available

Crystallization (Commentary)

Crystallization (Comment) Organism
crystallization in presence of (NH4)2SO4 Pseudomonas sp.

General Stability

General Stability Organism
2-mercaptoethanol stabilizes Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline weak, 2-mercaptoethanol partially protects Pseudomonas sp.
2,2'-dipyridyl 2-mercaptoethanol partially protects Pseudomonas sp.
Ag2+ 2-mercaptoethanol partially protects Pseudomonas sp.
Cd2+ 2-mercaptoethanol partially protects Pseudomonas sp.
Co2+ 2-mercaptoethanol partially protects Pseudomonas sp.
Cu2+ 2-mercaptoethanol partially protects Pseudomonas sp.
Hg2+ 2-mercaptoethanol partially protects Pseudomonas sp.
iodoacetamide 2-mercaptoethanol partially protects Pseudomonas sp.
Mersalyl acid strong Pseudomonas sp.
Mg2+ weak Pseudomonas sp.
N-ethylmaleimide 2-mercaptoethanol partially protects Pseudomonas sp.
NaF 2-mercaptoethanol partially protects Pseudomonas sp.
NaNO3 2-mercaptoethanol partially protects Pseudomonas sp.
Ni2+ weak Pseudomonas sp.
p-chloromercuribenzoate strong Pseudomonas sp.
p-chloromercuriphenylsulfonic acid strong Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
83
-
3-Cyano-L-alanine pH 9.5, 37°C Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
30 * 35000, SDS-PAGE Pseudomonas sp.
1000000
-
ultracentrifugation Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-cyano-L-alanine + H2O Pseudomonas sp.
-
L-aspartate + NH3 L-asparagine is formed as an intermediate ?
3-cyano-L-alanine + H2O Pseudomonas sp. 13
-
L-aspartate + NH3 L-asparagine is formed as an intermediate ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. 13
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
65
-
-
Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-cyano-L-alanine + H2O
-
Pseudomonas sp. L-aspartate + NH3 L-asparagine is formed as an intermediate ?
3-cyano-L-alanine + H2O
-
Pseudomonas sp. 13 L-aspartate + NH3 L-asparagine is formed as an intermediate ?
additional information no substrates are various nitrile or amide compounds, overview Pseudomonas sp. ?
-
?
additional information no substrates are various nitrile or amide compounds, overview Pseudomonas sp. 13 ?
-
?

Subunits

Subunits Comment Organism
polymer 30 * 35000, SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Pseudomonas sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 62 about half-maximal activity at 30°C and 62°C Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
stable up to for 10 min in 50 mM phosphate buffer, pH 7.5 Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
Tris-HCl buffer Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
8.2 10.8 about half-maximal activity at pH 8.2 and pH 10.8 Pseudomonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.5
-
3 h, stable at 37°C, only in the presence of 2-mercaptoethanol Pseudomonas sp.