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Literature summary for 3.5.5.1 extracted from

  • Yusuf, F.; Rather, I.A.; Jamwal, U.; Gandhi, S.G.; Chaubey, A.
    Cloning and functional characterization of nitrilase from Fusarium proliferatum AUF-2 for detoxification of nitriles (2015), Funct. Integr. Genomics, 15, 413-424 .
    View publication on PubMed
Search for more literature for 3.5.5.1

Activating Compound

Activating Compound Comment Organism Structure
EDTA 1 mM, 1.3fold stimulation Fusarium proliferatum

Cloned(Commentary)

Cloned (Comment) Organism
expression of the recombinant protein with His6-tag in Escherichia coli strain BL21 Fusarium proliferatum

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1 mM, complete inhibition Fusarium proliferatum
Ba2+ 1 mM, 45% inhibition Fusarium proliferatum
Co2+ 1 mM, 35% inhibition Fusarium proliferatum
Cu2+ 1 mM, 30% inhibition Fusarium proliferatum
Dichloromethane 5% (v/v), 70% inhibition Fusarium proliferatum
ethanol 5% (v/v), 45% inhibition Fusarium proliferatum
hexane 5% (v/v), 80% inhibition Fusarium proliferatum
Li+ 1 mM, 20% inhibition Fusarium proliferatum
methanol 5% (v/v), 30% inhibition Fusarium proliferatum
Ni2+ 1 mM, 40% inhibition Fusarium proliferatum
Propanol 5% (v/v), 80% inhibition Fusarium proliferatum
Toluene 5% (v/v), 95% inhibition Fusarium proliferatum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.55
-
 Benzonitrile pH 8.0, 37°C Fusarium proliferatum

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1 mM, 1.1.5fold stimulation Fusarium proliferatum
Fe2+ 1 mM, 1.1fold stimulation Fusarium proliferatum
Fe3+ 1 mM, 1.25fold stimulation Fusarium proliferatum
Mg2+ 1 mM, 1.25fold stimulation Fusarium proliferatum
Mn2+ 1 mM, 1.2fold stimulation Fusarium proliferatum
Zn2+ 1 mM, 1.2fold stimulation Fusarium proliferatum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
 SDS-PAGE Fusarium proliferatum

Organism

Organism UniProt Comment Textmining
Fusarium proliferatum A0A0E3D8K7
-
-
Fusarium proliferatum AUF-2 A0A0E3D8K7
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Fusarium proliferatum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
14.6
-
 pH 8.0, 37°C, substrate: benzonitrile Fusarium proliferatum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-cyanopyridine + 2 H2O 110% of the activity compared to benzonitrile Fusarium proliferatum pyridine 2-carboxylic acid + NH3
-
 ?
3-aminobutyronitrile + H2O 201% of the activity compared to benzonitrile Fusarium proliferatum 3-aminobutyric acid + NH3
-
 ?
3-cyanopyridine + 2 H2O 151% of the activity compared to benzonitrile Fusarium proliferatum pyridine-3-carboxylic acid + NH3
-
 ?
3-nitrobenzonitrile + H2O 110% of the activity compared to benzonitrile Fusarium proliferatum 3-nitrobenzoic acid + NH3
-
 ?
3-phenylpropionitrile + H2O 151% of the activity compared to benzonitrile Fusarium proliferatum 3-phenylpropionate + NH3
-
 ?
4-aminobutyronitrile + H2O 251% of the activity compared to benzonitrile Fusarium proliferatum 4-aminobutyric acid + NH3
-
 ?
4-cyanopyridine + 2 H2O 225% of the activity compared to benzonitrile Fusarium proliferatum pyridine-4-carboxylic acid + NH3
-
 ?
4-nitrobenzonitrile + H2O 151% of the activity compared to benzonitrile Fusarium proliferatum 4-nitrobenzoic acid + NH3
-
 ?
acetonitrile + H2O 135% of the activity compared to benzonitrile Fusarium proliferatum acetate + NH3
-
 ?
acetonitrile + H2O 135% of the activity compared to benzonitrile Fusarium proliferatum AUF-2 acetate + NH3
-
 ?
acrylonitrile + H2O 400% of the activity compared to benzonitrile Fusarium proliferatum acrylate + NH3
-
 ?
benzonitrile + 2 H2O
-
 Fusarium proliferatum benzoic acid + NH3
-
 ?
benzonitrile + 2 H2O
-
 Fusarium proliferatum AUF-2 benzoic acid + NH3
-
 ?
butyronitrile + H2O 425% of the activity compared to benzonitrile Fusarium proliferatum butyrate + NH3
-
 ?
butyronitrile + H2O 425% of the activity compared to benzonitrile Fusarium proliferatum AUF-2 butyrate + NH3
-
 ?
di-phenylacetonitrile + H2O 72% of the activity compared to benzonitrile Fusarium proliferatum ? + NH3
-
 ?
glutaronitrile + H2O 319% of the activity compared to benzonitrile Fusarium proliferatum ? + NH3
-
 ?
mandelonitrile + H2O 69% of the activity compared to benzonitrile Fusarium proliferatum ? + NH3
-
 ?
additional information no activity with fumaronitrile, succinonitrile Fusarium proliferatum ?
-
-
additional information no activity with fumaronitrile, succinonitrile Fusarium proliferatum AUF-2 ?
-
-
propionitrile + H2O 416% of the activity compared to benzonitrile Fusarium proliferatum propionate + NH3
-
 ?
propionitrile + H2O 416% of the activity compared to benzonitrile Fusarium proliferatum AUF-2 propionate + NH3
-
 ?

Subunits

Subunits Comment Organism
? x * 37000, SDS-PAGE Fusarium proliferatum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35 40
-
 Fusarium proliferatum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 45 20°C: about 40% of maximal activity, 45°C: about 60% of maximal activity Fusarium proliferatum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Fusarium proliferatum

pH Range

pH Minimum pH Maximum Comment Organism
6 9.5 pH 6.0: about 65% of maximal activity, pH 9.5: about 65% of maximal activity Fusarium proliferatum