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Literature summary for 3.5.5.1 extracted from
- Cloning, overexpression, and characterization of a thermostable nitrilase from an antarctic Pyrococcus sp (2017), Extremophiles, 21, 861-869 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| DTT | 1 mM | Pyrococcus sp. MC-FB |  |
| L-cysteine | 1 mM | Pyrococcus sp. MC-FB | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Pyrococcus sp. MC-FB |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Pyrococcus sp. MC-FB | |
| Cu2+ | - |
Pyrococcus sp. MC-FB | |
| Hg2+ | - |
Pyrococcus sp. MC-FB | |
| Zn2+ | - |
Pyrococcus sp. MC-FB | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 13.9 | - |
2-Cyanopyridine | pH 7.0, 90°C | Pyrococcus sp. MC-FB | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
gel filtration | Pyrococcus sp. MC-FB |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus sp. MC-FB | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pyrococcus sp. MC-FB |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0037 | - |
pH 7.0, 90°C, substrate: 2-cyanopyridine | Pyrococcus sp. MC-FB |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-chlorobenzonitrile + H2O | 1% of the activity compared to 2-cyanopyridine | Pyrococcus sp. MC-FB | 2-chlorobenzoic acid + NH3 | - |
? | |
| 2-cyanopyridine + 2 H2O | - |
Pyrococcus sp. MC-FB | pyridine-2-carboxylic acid + NH3 | - |
? | |
| 2-methylglutaronitrile + 4 H2O | 9% of the activity compared to 2-cyanopyridine | Pyrococcus sp. MC-FB | 2-methylglutaric acid + 2 NH3 | - |
? | |
| 3-chlorobenzonitrile + H2O | 14% of the activity compared to 2-cyanopyridine | Pyrococcus sp. MC-FB | 3-chlorobenzoic acid + NH3 | - |
? | |
| 3-cyanopyridine + 2 H2O | 30% of the activity compared to 2-cyanopyridine | Pyrococcus sp. MC-FB | pyridine-3-carboxylic acid + NH3 | - |
? | |
| 3-nitrobenzonitrile + H2O | 16% of the activity compared to 2-cyanopyridine | Pyrococcus sp. MC-FB | 3-nitrobenzoic acid + NH3 | - |
? | |
| 4-chlorobenzonitrile + H2O | 11% of the activity compared to 2-cyanopyridine | Pyrococcus sp. MC-FB | 4-chlorobenzoic acid + NH3 | - |
? | |
| 4-nitrobenzonitrile + H2O | 11% of the activity compared to 2-cyanopyridine | Pyrococcus sp. MC-FB | 4-nitrobenzoic acid + NH3 | - |
? | |
| adiponitrile + H2O | 3% of the activity compared to 2-cyanopyridine | Pyrococcus sp. MC-FB | adipic acid + NH3 | - |
? | |
| benzonitrile + 2 H2O | 7% of the activity compared to 2-cyanopyridine | Pyrococcus sp. MC-FB | benzoic acid + NH3 | - |
? | |
| additional information | the enzyme preferentially hydrolyzes aromatic nitriles. No activity with: cyclohexanecarbonitrile, malononitrile, butyronitrile, (R,S)-mandelonitrile, 2-phenylglycinonitrile, 2-nitrobenzonitrile | Pyrococcus sp. MC-FB | ? | - |
- |
|
| succinonitrile + H2O | 18% of the activity compared to 2-cyanopyridine | Pyrococcus sp. MC-FB | succinic acid + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 30000, SDS-PAGE | Pyrococcus sp. MC-FB |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| nitmc-fb | - |
Pyrococcus sp. MC-FB |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
- |
Pyrococcus sp. MC-FB |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | 100 | 85°C: about 45% of maximal activity, 100°C: about 50% of maximal activity | Pyrococcus sp. MC-FB |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
half-life: 8 h | Pyrococcus sp. MC-FB |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Pyrococcus sp. MC-FB |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7.5 | pH 6.0: about 60% of maximal activity, pH 7.5: about 50% of maximal activity | Pyrococcus sp. MC-FB |
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