Activating Compound | Comment | Organism | Structure |
---|---|---|---|
acetone | 5% (v/v), 1.2fold activation | Pannonibacter carbonis | |
dithiothreitol | 1 mM, 1.2fold activation | Pannonibacter carbonis | |
EDTA | 1 mM, 1.35fold activation | Pannonibacter carbonis | |
glycerin | 1% (v/v), 1.3fold activation | Pannonibacter carbonis | |
L-cysteine | 1 mM, 1.3fold activation | Pannonibacter carbonis | |
L-glutathione | 1 mM, 1.3fold activation | Pannonibacter carbonis | |
SDS | 1% (v/v), 1.4fold activation | Pannonibacter carbonis | |
Span-80 | 1% (v/v), 1.2fold activation | Pannonibacter carbonis |
Application | Comment | Organism |
---|---|---|
synthesis | the broad range of substrate specificity, the high activity towards aliphatic, aromatic, and arylacetonitriles, and the highly stability, in terms of temperature, pH, and metal ions make the enzyme a promising biocatalyst for mild nitrile hydrolysis | Pannonibacter carbonis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 (DE3) | Pannonibacter carbonis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
BaCl2 | 5 mM, 35% inhibition | Pannonibacter carbonis | |
CaCl2 | 5 mM, 34% inhibition | Pannonibacter carbonis | |
dimethyl sulfoxide | 1% (v/v) | Pannonibacter carbonis | |
dithiothreitol | 5 mM, 37% inhibition | Pannonibacter carbonis | |
DMSO | 5% (v/v), % inhibition | Pannonibacter carbonis | |
ether | 5% (v/v), 23% inhibition | Pannonibacter carbonis | |
ethyl acetate | 5% (v/v), 35% inhibition | Pannonibacter carbonis | |
FeCl3 | 5 mM, 23% inhibition | Pannonibacter carbonis | |
FeSO4 | 5 mM, 47% inhibition | Pannonibacter carbonis | |
glycerin | 5% (v/v), 52% inhibition | Pannonibacter carbonis | |
KCl | 5 mM, 58% inhibition | Pannonibacter carbonis | |
methanol | 5% (v/v), 29% inhibition | Pannonibacter carbonis | |
MgCl2 | 5 mM, 18% inhibition | Pannonibacter carbonis | |
n-heptane | 5% (v/v), 25% inhibition | Pannonibacter carbonis | |
NaCl | 5 mM, 26% inhibition | Pannonibacter carbonis | |
SDS | 5% (v/v), 37% inhibition | Pannonibacter carbonis | |
Triton X-100 | 5% (v/v), 14% inhibition | Pannonibacter carbonis | |
ZnCl2 | 5 mM, 32% inhibition | Pannonibacter carbonis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.31 | - |
3-Cyanopyridine | pH 7.0, 65°C | Pannonibacter carbonis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 1 mM, 3.2fold activation | Pannonibacter carbonis | |
Cr3+ | 5 mM, 2.8fold activation | Pannonibacter carbonis | |
Cu2+ | 5 mM, 1.3fold activation | Pannonibacter carbonis | |
Hg2+ | 5 mM, 5.2fold activation | Pannonibacter carbonis | |
Ni2+ | 5 mM, 3fold activation | Pannonibacter carbonis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pannonibacter carbonis | - |
- |
- |
Pannonibacter carbonis Q4.6 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pannonibacter carbonis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methylglutaronitrile + H2O | - |
Pannonibacter carbonis | ? + NH3 | - |
? | |
3-cyanopyridine + 2 H2O | - |
Pannonibacter carbonis | nicotinic acid + NH3 | - |
? | |
4-chlorobutyronitrile + H2O | - |
Pannonibacter carbonis | 4-chlorobutyrate + NH3 | - |
? | |
acetonitrile + H2O | - |
Pannonibacter carbonis | acetic acid + NH3 | - |
? | |
acrylonitrile + H2O | - |
Pannonibacter carbonis | acrylic acid + NH3 | - |
? | |
benzonitrile + 2 H2O | - |
Pannonibacter carbonis | benzoic acid + NH3 | - |
? | |
dicyanobutane + H2O | - |
Pannonibacter carbonis | ? + NH3 | - |
? | |
dicyanooctane + H2O | - |
Pannonibacter carbonis | ? + NH3 | - |
? | |
dodecanenitrile + H2O | - |
Pannonibacter carbonis | dodecanoic acid + NH3 | - |
? | |
fumaronitrile + H2O | - |
Pannonibacter carbonis | fumaric acid + NH3 | - |
? | |
iminodiacetonitrile + H2O | - |
Pannonibacter carbonis | iminodiacetic acid + NH3 | - |
? | |
indole-3-acetonitrile + H2O | - |
Pannonibacter carbonis | indol-3-acetic acid + NH3 | - |
? | |
mandelonitrile + H2O | - |
Pannonibacter carbonis | mandelic acid + NH3 | - |
? | |
pentanenitrile + H2O | - |
Pannonibacter carbonis | pentanoic acid + NH3 | - |
? | |
phenylacetonitrile + 2 H2O | - |
Pannonibacter carbonis | phenylacetic acid + NH3 | - |
? | |
phenylenediacetonitrile + H2O | - |
Pannonibacter carbonis | ? + NH3 | - |
? | |
phenylpropionitrile + 2 H2O | - |
Pannonibacter carbonis | phenylpropionic acid + NH3 | - |
? | |
succinonitrile + H2O | - |
Pannonibacter carbonis | succinic acid + NH3 | - |
? | |
thiophene + H2O | - |
Pannonibacter carbonis | ? + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PaCNit | - |
Pannonibacter carbonis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Pannonibacter carbonis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 90 | 30°C: about 60% of maximal activity, 90°C: about 50% of maximal activity | Pannonibacter carbonis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Pannonibacter carbonis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 11 | pH 4.0: about 40% of maximal activity, pH 11.0: about 50% of maximal activity | Pannonibacter carbonis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | relative enzyme activity is retained at pH 6.0 and 7.0 after incubating for 12 h at 4°C | Pannonibacter carbonis |