Protein Variants | Comment | Organism |
---|---|---|
A198V | the mutant enzyme shows 2.4fold increased conversion rate of 2-phenylpropionitrile at pH 7.5 compared to the wild type enzyme | Alcaligenes faecalis |
A198V/I290F | the mutant enzyme shows 1.2fold increased conversion rate of 2-phenylpropionitrile at pH 7.5 and 140% activity at pH 4.5 compared to the wild type enzyme | Alcaligenes faecalis |
A198V/I290F/H135Y | the mutant enzyme shows 1.7fold increased conversion rate of 2-phenylpropionitrile at pH 7.5 and 150% activity at pH 4.5 compared to the wild type enzyme | Alcaligenes faecalis |
A198V/I290F/H135Y/Y213H/T350S/Y177C/A285T | the mutant enzyme shows 2.8fold increased conversion rate of 2-phenylpropionitrile at pH 7.5 and 740% activity at pH 4.5 compared to the wild type enzyme | Alcaligenes faecalis |
A198V/I290F/Y177C/A285T | the mutant enzyme shows 0.9fold conversion rate of 2-phenylpropionitrile at pH 7.5 and 240% activity at pH 4.5 compared to the wild type enzyme | Alcaligenes faecalis |
A198V/I290F/Y213H/T350S | the mutant enzyme shows 2fold increased conversion rate of 2-phenylpropionitrile at pH 7.5 and 200% activity at pH 4.5 compared to the wild type enzyme | Alcaligenes faecalis |
A198V/Q197H | the mutant enzyme shows 2.9fold increased conversion rate of 2-phenylpropionitrile at pH 7.5 compared to the wild type enzyme | Alcaligenes faecalis |
A198V/Q197H/L176M | the mutant enzyme shows 6.5fold increased conversion rate of 2-phenylpropionitrile at pH 7.5 compared to the wild type enzyme | Alcaligenes faecalis |
A198V/Q197H/L176M/V306I | the mutant enzyme shows 8fold increased conversion rate of 2-phenylpropionitrile at pH 7.5 compared to the wild type enzyme | Alcaligenes faecalis |
I290F/Q3L | the mutant enzyme shows 2.3fold increased conversion rate of 2-phenylpropionitrile at pH 7.5 and 100% activity at pH 4.5 compared to the wild type enzyme | Alcaligenes faecalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alcaligenes faecalis | P20960 | - |
- |
Alcaligenes faecalis JM3 | P20960 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni Sepharose column chromatography | Alcaligenes faecalis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.1 | - |
the specific activity is less than 0.1 micromol/min/mg, wild type enzyme, using (R)-2-chloromandelonitrile as substrate, in 50 mM potassium phosphate buffer, at pH 4.5 and 30°C | Alcaligenes faecalis |
0.14 | - |
mutant enzyme A198V/I290F/H135Y/Y213H/T350S/Y177C/A285T, using (R)-2-chloromandelonitrile as substrate, in 50 mM potassium phosphate buffer, at pH 7.5 and 30°C | Alcaligenes faecalis |
0.52 | - |
mutant enzyme A198V/I290F/H135Y/Y213H/T350S/Y177C/A285T, using (R)-2-chloromandelonitrile as substrate, in 50 mM potassium phosphate buffer, at pH 4.5 and 30°C | Alcaligenes faecalis |
0.62 | - |
wild type enzyme, using (R)-2-chloromandelonitrile as substrate, in 50 mM potassium phosphate buffer, at pH 7.5 and 30°C | Alcaligenes faecalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-2-chloromandelonitrile + H2O | - |
Alcaligenes faecalis | (R)-2-chloromandelic acid + (S)-2-chloromandelic acid + NH3 | the wild type enzyme yields 76% (R)-2-chloromandelic acid and 9% (S)-2-chloromandelic acid at pH 7.5, as well as 12% (R)-2-chloromandelic acid and no (S)-2-chloromandelic acid at pH 4.5 | ? | |
(R)-2-chloromandelonitrile + H2O | - |
Alcaligenes faecalis JM3 | (R)-2-chloromandelic acid + (S)-2-chloromandelic acid + NH3 | the wild type enzyme yields 76% (R)-2-chloromandelic acid and 9% (S)-2-chloromandelic acid at pH 7.5, as well as 12% (R)-2-chloromandelic acid and no (S)-2-chloromandelic acid at pH 4.5 | ? | |
2-phenylpropionitrile + 2 H2O | - |
Alcaligenes faecalis | 2-phenylpropionic acid + NH3 | - |
? | |
2-phenylpropionitrile + 2 H2O | - |
Alcaligenes faecalis JM3 | 2-phenylpropionic acid + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NIT | - |
Alcaligenes faecalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
wild type enzyme | Alcaligenes faecalis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 7.5 | the wild type enzyme shows no activity after 30 min incubation at pH 4.5, about 10% activity after 30 min incubation at pH 5.0, about 20% activity after 30 min incubation at pH 5.5, about 60% activity after 30 min incubation at pH 6.0, and 100% activity after 30 min incubation at pH 6.5-7.5 | Alcaligenes faecalis |