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Literature summary for 3.5.5.1 extracted from
- Purification and characterization of intracellular nitrilases from Rhodococcus sp. - potential role of periplasmic nitrilase (2010), Afr. J. Microbiol. Res., 4, 1148-1153.
No PubMed abstract available
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Rhodococcus rhodochrous | 5737 | - |
| cytoplasm | - |
Rhodococcus fascians | 5737 | - |
| periplasm | - |
Rhodococcus rhodochrous | - |
- |
| periplasm | - |
Rhodococcus fascians | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodococcus fascians | - |
- |
- |
| Rhodococcus fascians MTCC-1531 | - |
- |
- |
| Rhodococcus rhodochrous | - |
- |
- |
| Rhodococcus rhodochrous MTCC-291 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzonitrile + 2 H2O | the substrate concentration of 40 mM is optimum for hydrolyzing benzonitrile | Rhodococcus rhodochrous | benzoic acid + NH3 | - |
? |  |
| benzonitrile + 2 H2O | the substrate concentration of 40 mM is optimum for hydrolyzing benzonitrile | Rhodococcus fascians | benzoic acid + NH3 | - |
? | |
| benzonitrile + 2 H2O | the substrate concentration of 40 mM is optimum for hydrolyzing benzonitrile | Rhodococcus fascians MTCC-1531 | benzoic acid + NH3 | - |
? | |
| benzonitrile + 2 H2O | the substrate concentration of 40 mM is optimum for hydrolyzing benzonitrile | Rhodococcus rhodochrous MTCC-291 | benzoic acid + NH3 | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Rhodococcus rhodochrous |
| 40 | - |
- |
Rhodococcus fascians |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 5 | 40 | nitrilase activity increases gradually from 5-40°C only, no further increase in activity is recorded above 40°C | Rhodococcus fascians |
| 5 | 50 | the enzyme activity increases gradually from 5-45°C and decreases drastically at 50°C | Rhodococcus rhodochrous |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
incubation at 45°C does not affect the activity of nitrilase enzyme. A gradual decrease in benzonitrile hydrolyzing activity is observed up to 4 h of pre-incubation at 45°C followed by no detectable enzyme activity | Rhodococcus rhodochrous |
| 45 | - |
the enzyme activity decreases up to 5 h at 45°c with no further detectable enzyme activity | Rhodococcus fascians |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Rhodococcus fascians |
| 8.5 | - |
- |
Rhodococcus rhodochrous |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
the enzyme retains about 68% activity at pH 9.5 | Rhodococcus rhodochrous |
| 9.5 | - |
the enzyme retains about 72% activity at pH 9.5 | Rhodococcus fascians |
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Release 2023.1 (January 2023)
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