Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 CodonPlus (DE3) RIL cells | Sorghum bicolor |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
recombinant isozyme NIT4A, SDS-PAGE | Sorghum bicolor |
42000 | - |
recombinant isozyme NIT4B2, SDS-PAGE | Sorghum bicolor |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sorghum bicolor | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and Ni-NTA column chromatography | Sorghum bicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phenylacetonitrile + 2 H2O | substrate for isozyme complex NIT4A/B2 | Sorghum bicolor | phenylacetate + NH3 | - |
? | |
4-hydroxyphenylacetonitrile + H2O | substrate for isozyme complex NIT4A/B2 | Sorghum bicolor | 4-hydroxyphenylacetic acid + NH3 | - |
? | |
6-heptenenitrile + H2O | substrate for isozyme NIT4A/B2 | Sorghum bicolor | 6-heptenoic acid + NH3 | - |
? | |
indole-3-acetonitrile + H2O | substrate for isozyme complex NIT4A/B2 | Sorghum bicolor | indol-3-acetic acid + NH3 | - |
? | |
additional information | recombinant NIT4 isozymes NIT4A, NIT4B1, and NIT4B2 do not hydrolyze 3-cyano-L-alanine, benzonitrile, 4-hydroxybenzonitrile, butyronitrile, and 4-pentenenitrile | Sorghum bicolor | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NIT4A/B1 | active isozyme complex | Sorghum bicolor |
NIT4A/B2 | actice isozyme complex | Sorghum bicolor |