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Literature summary for 3.5.5.1 extracted from

  • Vejvoda, V.; Kaplan, O.; Bezouska, K.; Pompach, P.; Sulc, M.; Cantarella, M.; Benada, O.; Uhnakova, B.; Rinagelova, A.; Lutz-Wahl, S.; Fischer, L.; Kren, V.; Martinkova, L.
    Purification and characterization of a nitrilase from Fusarium solani O1 (2008), J. Mol. Catal. B, 50, 99-106.
No PubMed abstract available
Search for more literature for 3.5.5.1

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1.5% residual activity at 1 mM Fusarium solani
Al3+ 0.3% residual activity at 5 mM Fusarium solani
Co2+ 46.3% residual activity at 5 mM Fusarium solani
Cu2+ 13.4% residual activity at 1 mM Fusarium solani
dithiothreitol 55% residual activity at 1 mM Fusarium solani
EDTA 87% residual activity at 1 mM Fusarium solani
Fe3+ 5% residual activity at 5 mM Fusarium solani
Hg2+ complete loss of activity at 1 mM Fusarium solani
iodoacetamide 5% residual activity at 4.6 mM Fusarium solani
Mg2+ 46% residual activity at 5 mM Fusarium solani
NaN3 38% residual activity at 5 mM Fusarium solani
Ni2+ 7% residual activity at 5 mM Fusarium solani
p-hydroxymercuribenzoate 12% residual activity at 1 mM Fusarium solani
Pb2+ 6% residual activity at 1 mM Fusarium solani
Zn2+ 6% residual activity at 5 mM Fusarium solani

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4
-
 4-cyanopyridine
-
 Fusarium solani
1.5
-
 Benzonitrile
-
 Fusarium solani
3.2
-
 3-Cyanopyridine
-
 Fusarium solani

Metals/Ions

Metals/Ions Comment Organism Structure
Cr3+ 4% increased activity at 1 mM Fusarium solani

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 x * 40000, gel filtration Fusarium solani
580000
-
 gel filtration Fusarium solani

Organic Solvent Stability

Organic Solvent Comment Organism
2-propanol the enzyme shows more than 30% of the control activity with 5% 2-propanol Fusarium solani
Acetone the enzyme shows more than 30% of the control activity with 5% acetone Fusarium solani
acetonitrile the enzyme shows more than 30% of the control activity with 5% acetonitrile Fusarium solani
dichloromethane the enzyme shows more than 30% of the control activity with 5% dichloromethane Fusarium solani
dimethylsulfoxide the enzyme shows more than 30% of the control activity with 5-10% of dimethylsulfoxide Fusarium solani
Ethanol more than half of activity is preserved at up to 15% of ethanol Fusarium solani
Ethyl acetate the enzyme shows more than 30% of the control activity with 5% ethyl acetate Fusarium solani
Methanol the enzyme shows more than 30% of the control activity with 5-10% of methanol Fusarium solani
n-heptane more than half of activity is preserved at up to 50% of n-heptane Fusarium solani
n-hexane more than half of activity is preserved at up to 50% of n-hexane Fusarium solani
toluene the enzyme shows more than 30% of the control activity with 5-10% of toluene Fusarium solani
xylene more than half of activity is preserved at up to 15% of xylene, the enzyme shows more than 30% of the control activity with 5-10% of xylene Fusarium solani

Organism

Organism UniProt Comment Textmining
Fusarium solani
-
-
-
Fusarium solani O1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Hi-Prep 16/10 Phenyl FF column chromatography, Q Sepharose column chromatography, Sephacryl S-200 gel filtration, and Superdex 200 gel filtration Fusarium solani

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Fusarium solani
-

Storage Stability

Storage Stability Organism
-18°C, in presence of 2% (w/v) sucrose as a stabilizer, 2 months, 8% loss of activity Fusarium solani
-80°C, in presence of 2% (w/v) sucrose as a stabilizer, 2 months, no significant loss of activity Fusarium solani

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-chlorobenzonitrile + H2O 87% activity compared to benzonitrile Fusarium solani 3-chlorobenzoic acid + NH3
-
 ?
3-chlorobenzonitrile + H2O 87% activity compared to benzonitrile Fusarium solani O1 3-chlorobenzoic acid + NH3
-
 ?
3-cyanopyridine + 2 H2O 28% activity compared to benzonitrile Fusarium solani 3-pyridinecarboxylic acid + NH3
-
 ?
3-hydroxybenzonitrile + H2O 80% activity compared to benzonitrile Fusarium solani 3-hydroxybenzoic acid + NH3
-
 ?
3-tolunitrile + H2O 33% activity compared to benzonitrile Fusarium solani 3-methylbenzoate + NH3
-
 ?
4-aminobenzonitrile + H2O 7% activity compared to benzonitrile Fusarium solani 4-aminobenzoic acid + NH3
-
 ?
4-chlorobenzonitrile + H2O 40% activity compared to benzonitrile Fusarium solani 4-chlorobenzoic acid + NH3
-
 ?
4-chlorobenzonitrile + H2O 40% activity compared to benzonitrile Fusarium solani O1 4-chlorobenzoic acid + NH3
-
 ?
4-cyanopyridine + 2 H2O 130% activity compared to benzonitrile Fusarium solani 4-pyridinecarboxylic acid + NH3
-
 ?
4-cyanopyridine + 2 H2O 130% activity compared to benzonitrile Fusarium solani O1 4-pyridinecarboxylic acid + NH3
-
 ?
4-hydroxybenzonitrile + H2O 3% activity compared to benzonitrile Fusarium solani 4-hydroxybenzoic acid + NH3
-
 ?
4-tolunitrile + H2O 16% activity compared to benzonitrile Fusarium solani 4-methylbenzoate + NH3
-
 ?
benzonitrile + 2 H2O 100% activity Fusarium solani benzoic acid + NH3
-
 ?
benzonitrile + 2 H2O 100% activity Fusarium solani O1 benzoic acid + NH3
-
 ?
butyronitrile + H2O 20% activity compared to benzonitrile Fusarium solani butyric acid + NH3
-
 ?
isobutyronitrile + H2O 4% activity compared to benzonitrile Fusarium solani isobutyric acid + NH3
-
 ?
lactonitrile + H2O 4% activity compared to benzonitrile Fusarium solani lactic acid + NH3
-
 ?
methacrylonitrile + H2O 14% activity compared to benzonitrile Fusarium solani methylacrylic acid + NH3
-
 ?
methacrylonitrile + H2O 14% activity compared to benzonitrile Fusarium solani O1 methylacrylic acid + NH3
-
 ?
propionitrile + H2O 18% activity compared to benzonitrile Fusarium solani propionic acid + NH3
-
 ?
valeronitrile + H2O 26% activity compared to benzonitrile Fusarium solani valeric acid + NH3
-
 ?

Subunits

Subunits Comment Organism
multimer x * 40000, gel filtration Fusarium solani

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 45
-
 Fusarium solani

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35 55 the nitrilase half-life is 277 min and 10.5 h at 35°C and 45°C, respectively, the enzyme is active at up to 50°C, the enzyme shows only traces of activity at 55°C Fusarium solani

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Fusarium solani

pH Range

pH Minimum pH Maximum Comment Organism
5 10
-
 Fusarium solani

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 11 the enzyme shows 5%, 39% and 29% of the maximum activity at pH 5, 6 and 10, respectively, and it is nearly inactive at pH 11 Fusarium solani