Protein Variants | Comment | Organism |
---|---|---|
F168I/L201E | 4.6fold increased specific activity compared to the wild type enzyme | Acidovorax facilis |
F168K | 3.1fold increased specific activity compared to the wild type enzyme | Acidovorax facilis |
F168M | 3fold increased specific activity compared to the wild type enzyme | Acidovorax facilis |
F168T | 1.7fold increased specific activity compared to the wild type enzyme | Acidovorax facilis |
F168T/L201Q | 3.1fold increased specific activity compared to the wild type enzyme | Acidovorax facilis |
F168V | 4.1fold increased specific activity compared to the wild type enzyme | Acidovorax facilis |
F168V/L201N | 15.3fold increased specific activity compared to the wild type enzyme | Acidovorax facilis |
L201A | 1.9fold increased specific activity compared to the wild type enzyme | Acidovorax facilis |
L201G | 1.5fold increased specific activity compared to the wild type enzyme | Acidovorax facilis |
L201H | 2.7fold increased specific activity compared to the wild type enzyme | Acidovorax facilis |
L201K | 3.5fold increased specific activity compared to the wild type enzyme | Acidovorax facilis |
L201N | 5.5fold increased specific activity compared to the wild type enzyme | Acidovorax facilis |
L201Q | 4.9fold increased specific activity compared to the wild type enzyme | Acidovorax facilis |
L201S | 2.2fold increased specific activity compared to the wild type enzyme | Acidovorax facilis |
L201T | 2fold increased specific activity compared to the wild type enzyme | Acidovorax facilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidovorax facilis | - |
- |
- |
Acidovorax facilis 72W | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzonitrile + 2 H2O | - |
Acidovorax facilis | benzoic acid + NH3 | - |
? | |
benzonitrile + 2 H2O | - |
Acidovorax facilis 72W | benzoic acid + NH3 | - |
? | |
glycolonitrile + H2O | - |
Acidovorax facilis | ammonium glycolate + ? | - |
? | |
glycolonitrile + H2O | - |
Acidovorax facilis 72W | ammonium glycolate + ? | - |
? |