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Literature summary for 3.5.5.1 extracted from
- The nitrilase family of CN hydrolyzing enzymes - a comparative study (2003), J. Appl. Microbiol., 95, 1161-1174.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination, genes of the 3 isozymes are clustered on chromosome 3 | Arabidopsis thaliana |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
x * 32000, strain ATCC 8750, x * 44000, strain JM3 | Alcaligenes faecalis |
| 37000 | - |
x * 37000 | Fusarium oxysporum |
| 38000 | - |
x * 38000 | Comamonas testosteroni |
| 40000 | - |
x * 40000 | Rhodococcus sp. |
| 40000 | - |
x * 40000, strain J1 | Rhodococcus rhodochrous |
| 41000 | - |
strain SI | Pseudomonas sp. |
| 41000 | - |
x * 41000 | Rhodococcus rhodochrous |
| 41000 | - |
x * 41000 + x * 72000, enzyme + groEL | Aeribacillus pallidus |
| 44000 | - |
x * 32000, strain ATCC 8750, x * 44000, strain JM3 | Alcaligenes faecalis |
| 45000 | - |
strain PA-34 | Rhodococcus rhodochrous |
| 45000 | - |
x * 45000, strain NCIB11216 and strain NCIB11215 | Nocardia sp. |
| 72000 | - |
x * 41000 + x * 72000, enzyme + groEL | Aeribacillus pallidus |
| 74000 | - |
- |
Klebsiella pneumoniae |
| 76000 | - |
x * 76000 | Fusarium solani |
| 260000 | - |
strain JM3 | Alcaligenes faecalis |
| 410000 | - |
strain J1 | Rhodococcus rhodochrous |
| 460000 | - |
strain ATCC 8750 | Alcaligenes faecalis |
| 550000 | - |
- |
Fusarium oxysporum |
| 560000 | - |
- |
Rhodococcus sp. |
| 560000 | - |
strain NCIB11216 and strain NCIB11215 | Nocardia sp. |
| 580000 | - |
- |
Acinetobacter sp. |
| 600000 | - |
- |
Aeribacillus pallidus |
| 620000 | - |
- |
Fusarium solani |
| 650000 | - |
- |
Rhodococcus rhodochrous |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| bromoxynil + H2O | Klebsiella pneumoniae | enzyme utilizes the herbicide substrate as nitrogen source | ? | - |
? |  |
| indole-3-acetonitrile + H2O | Arabidopsis thaliana | isozymes are involved in indole-3-acetic acid production | indole-3-acetic acid + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acidovorax facilis | - |
- |
- |
| Acidovorax facilis 72W | - |
- |
- |
| Acinetobacter sp. | - |
- |
- |
| Acinetobacter sp. AK226 | - |
- |
- |
| Aeribacillus pallidus | - |
- |
- |
| Aeribacillus pallidus Dac521 | - |
- |
- |
| Alcaligenes faecalis | - |
and ATCC 8750 | - |
| Alcaligenes faecalis JM3 | - |
and ATCC 8750 | - |
| Arabidopsis thaliana | - |
3 isoenzymes NIT1-3 | - |
| Bacillus sp. (in: Bacteria) | - |
- |
- |
| Bacillus sp. (in: Bacteria) OxB-1 | - |
- |
- |
| Brassica rapa | - |
ssp. pehinensis, chinese cabbage | - |
| Comamonas testosteroni | - |
- |
- |
| Fusarium oxysporum | - |
f.sp. melonis | - |
| Fusarium solani | - |
- |
- |
| Fusarium solani IMI196840 | - |
- |
- |
| Hordeum vulgare | - |
- |
- |
| Klebsiella pneumoniae | - |
ssp. ozaenae | - |
| Nocardia sp. | - |
i.e. Rhodococcus sp. strain NCIB11216 and NCIB11215 | - |
| Pseudomonas sp. | - |
strain DSM 7155 and strain SI | - |
| Rhodococcus rhodochrous | - |
- |
- |
| Rhodococcus rhodochrous | - |
strains PA-34 and J1 | - |
| Rhodococcus rhodochrous K22 | - |
- |
- |
| Rhodococcus sp. | - |
strain ATCC39484 | - |
| Rhodococcus sp. ATCC 39484 | - |
strain ATCC39484 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Klebsiella pneumoniae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,4-benzodinitrile + H2O | - |
Fusarium solani | ? | - |
? | |
| 2-bromobenzonitrile + H2O | best substrate of strain NCIB11216 and strain NCIB11215 | Nocardia sp. | 2-bromobenzoic acid + NH3 | - |
? | |
| 2-furanocarbonitrile + H2O | - |
Rhodococcus sp. | 2-furanocarboxylic acid + NH3 | - |
? | |
| 2-furanocarbonitrile + H2O | - |
Rhodococcus sp. ATCC 39484 | 2-furanocarboxylic acid + NH3 | - |
? | |
| 2-nitrobenzonitrile + H2O | strain NCIB11215 | Nocardia sp. | 2-nitrobenzoic acid + NH3 | - |
? | |
| 2-thiophenacetonitrile + H2O | strain JM3 | Alcaligenes faecalis | 2-thiophenacetic acid + NH3 | - |
? | |
| 2-thiophenacetonitrile + H2O | strain JM3 | Alcaligenes faecalis JM3 | 2-thiophenacetic acid + NH3 | - |
? | |
| acetonitrile + H2O | strain SI | Pseudomonas sp. | acetic acid + NH3 | - |
? | |
| acrylonitrile + H2O | - |
Rhodococcus rhodochrous | acrylic acid + NH3 | - |
? | |
| acrylonitrile + H2O | - |
Comamonas testosteroni | acrylic acid + NH3 | - |
? | |
| acrylonitrile + H2O | - |
Fusarium solani | acrylic acid + NH3 | - |
? | |
| acrylonitrile + H2O | - |
Fusarium oxysporum | acrylic acid + NH3 | - |
? | |
| acrylonitrile + H2O | best substrate | Rhodococcus rhodochrous | acrylic acid + NH3 | - |
? | |
| acrylonitrile + H2O | low activity | Aeribacillus pallidus | acrylic acid + NH3 | - |
? | |
| acrylonitrile + H2O | high activity | Acinetobacter sp. | acrylic acid + NH3 | - |
? | |
| acrylonitrile + H2O | best substrate of strain SI | Pseudomonas sp. | acrylic acid + NH3 | - |
? | |
| acrylonitrile + H2O | - |
Fusarium solani IMI196840 | acrylic acid + NH3 | - |
? | |
| acrylonitrile + H2O | high activity | Acinetobacter sp. AK226 | acrylic acid + NH3 | - |
? | |
| acrylonitrile + H2O | - |
Rhodococcus rhodochrous K22 | acrylic acid + NH3 | - |
? | |
| acrylonitrile + H2O | best substrate | Rhodococcus rhodochrous K22 | acrylic acid + NH3 | - |
? | |
| acrylonitrile + H2O | - |
Fusarium solani IMI 196840 | acrylic acid + NH3 | - |
? | |
| acrylonitrile + H2O | low activity | Aeribacillus pallidus Dac521 | acrylic acid + NH3 | - |
? | |
| adiponitrile + H2O | - |
Comamonas testosteroni | adipic acid + NH3 | - |
? | |
| benzonitrile + 2 H2O | - |
Rhodococcus rhodochrous | benzoic acid + NH3 | - |
? | |
| benzonitrile + 2 H2O | - |
Rhodococcus sp. | benzoic acid + NH3 | - |
? | |
| benzonitrile + 2 H2O | - |
Acinetobacter sp. | benzoic acid + NH3 | - |
? | |
| benzonitrile + 2 H2O | - |
Fusarium oxysporum | benzoic acid + NH3 | - |
? | |
| benzonitrile + 2 H2O | - |
Aeribacillus pallidus | benzoic acid + NH3 | - |
? | |
| benzonitrile + 2 H2O | very low activity | Klebsiella pneumoniae | benzoic acid + NH3 | - |
? | |
| benzonitrile + 2 H2O | low activity | Rhodococcus rhodochrous | benzoic acid + NH3 | - |
? | |
| benzonitrile + 2 H2O | low activity | Comamonas testosteroni | benzoic acid + NH3 | - |
? | |
| benzonitrile + 2 H2O | low activity | Fusarium solani | benzoic acid + NH3 | - |
? | |
| benzonitrile + 2 H2O | high activity in strain NCIB11216 and strain NCIB11215 | Nocardia sp. | benzoic acid + NH3 | - |
? | |
| benzonitrile + 2 H2O | strain ATCC 8750 | Alcaligenes faecalis | benzoic acid + NH3 | - |
? | |
| benzonitrile + 2 H2O | strain SI, low activity | Pseudomonas sp. | benzoic acid + NH3 | - |
? | |
| benzonitrile + 2 H2O | low activity | Fusarium solani IMI196840 | benzoic acid + NH3 | - |
? | |
| benzonitrile + 2 H2O | - |
Rhodococcus sp. ATCC 39484 | benzoic acid + NH3 | - |
? | |
| benzonitrile + 2 H2O | - |
Acinetobacter sp. AK226 | benzoic acid + NH3 | - |
? | |
| benzonitrile + 2 H2O | - |
Rhodococcus rhodochrous K22 | benzoic acid + NH3 | - |
? | |
| benzonitrile + 2 H2O | low activity | Rhodococcus rhodochrous K22 | benzoic acid + NH3 | - |
? | |
| benzonitrile + 2 H2O | low activity | Fusarium solani IMI 196840 | benzoic acid + NH3 | - |
? | |
| benzonitrile + 2 H2O | strain ATCC 8750 | Alcaligenes faecalis JM3 | benzoic acid + NH3 | - |
? | |
| benzonitrile + 2 H2O | - |
Aeribacillus pallidus Dac521 | benzoic acid + NH3 | - |
? | |
| bromoxynil + H2O | enzyme utilizes the herbicide substrate as nitrogen source | Klebsiella pneumoniae | ? | - |
? | |
| bromoxynil + H2O | 3',5-dihalogenated 4-hydroxybenzonitrile compound, high activity, substrate is a herbicide | Klebsiella pneumoniae | ? | - |
? | |
| bromoxynil + H2O | strain NCIB11215, very low activity, 3',5-dihalogenated 4-hydroxybenzonitrile compound | Nocardia sp. | ? | - |
? | |
| chloroxynil + H2O | 3',5-dihalogenated 4-hydroxybenzonitrile compound | Klebsiella pneumoniae | ? | - |
? | |
| crotononitrile + 2 H2O | - |
Aeribacillus pallidus | crotonic acid + NH3 | - |
? | |
| crotononitrile + 2 H2O | - |
Aeribacillus pallidus Dac521 | crotonic acid + NH3 | - |
? | |
| crotononitrile + H2O | - |
Rhodococcus rhodochrous | ? | - |
? | |
| crotononitrile + H2O | - |
Fusarium oxysporum | ? | - |
? | |
| indole-3-acetonitrile + H2O | - |
Arabidopsis thaliana | indole-3-acetic acid + NH3 | - |
? | |
| indole-3-acetonitrile + H2O | isozymes are involved in indole-3-acetic acid production | Arabidopsis thaliana | indole-3-acetic acid + NH3 | - |
? | |
| ioxynil + H2O | 3',5-dihalogenated 4-hydroxybenzonitrile compound | Klebsiella pneumoniae | ? | - |
? | |
| ioxynil + H2O | 3',5-dihalogenated 4-hydroxybenzonitrile compound, strain NCIB11215 | Nocardia sp. | ? | - |
? | |
| mandelonitrile + 2 H2O | best substrate of strain ATCC 8750, low activity in strain JM3 | Alcaligenes faecalis | mandelic acid + NH3 | - |
? | |
| mandelonitrile + 2 H2O | best substrate of strain ATCC 8750, low activity in strain JM3 | Alcaligenes faecalis JM3 | mandelic acid + NH3 | - |
? | |
| additional information | substrate specificity | Hordeum vulgare | ? | - |
? | |
| additional information | substrate specificity | Brassica rapa | ? | - |
? | |
| additional information | substrate specificity, overview | Alcaligenes faecalis | ? | - |
? | |
| additional information | substrate specificity, overview | Rhodococcus rhodochrous | ? | - |
? | |
| additional information | substrate specificity, overview | Comamonas testosteroni | ? | - |
? | |
| additional information | substrate specificity, overview | Nocardia sp. | ? | - |
? | |
| additional information | substrate specificity, overview | Acinetobacter sp. | ? | - |
? | |
| additional information | substrate specificity, overview | Acidovorax facilis | ? | - |
? | |
| additional information | substrate specificity, overview | Fusarium solani | ? | - |
? | |
| additional information | substrate specificity, overview | Fusarium oxysporum | ? | - |
? | |
| additional information | substrate specificity, overview | Aeribacillus pallidus | ? | - |
? | |
| additional information | stricter substrate specificity compared to nitrilases from other species, strong preference for 4-hydroxybenzonitriles | Klebsiella pneumoniae | ? | - |
? | |
| additional information | substrate specificity, enzyme catalyzes a range of 2-pyridones with rates of 1-118% relative to ricinine, overview | Pseudomonas sp. | ? | - |
? | |
| additional information | substrate specificity, overview | Fusarium solani IMI196840 | ? | - |
? | |
| additional information | substrate specificity, overview | Acinetobacter sp. AK226 | ? | - |
? | |
| additional information | substrate specificity, overview | Rhodococcus rhodochrous K22 | ? | - |
? | |
| additional information | substrate specificity, overview | Fusarium solani IMI 196840 | ? | - |
? | |
| additional information | substrate specificity, overview | Alcaligenes faecalis JM3 | ? | - |
? | |
| additional information | substrate specificity, overview | Aeribacillus pallidus Dac521 | ? | - |
? | |
| additional information | substrate specificity, overview | Acidovorax facilis 72W | ? | - |
? | |
| N-methyl-3-cyano-4-methoxy-2-pyridone + H2O | - |
Pseudomonas sp. | ? | - |
? | |
| p-aminobenzyl cyanide + H2O | strains ATCC 8750 and JM3 | Alcaligenes faecalis | ? | - |
? | |
| p-aminobenzyl cyanide + H2O | strains ATCC 8750 and JM3 | Alcaligenes faecalis JM3 | ? | - |
? | |
| ricinine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40000 | Rhodococcus sp. |
| ? | x * 32000, strain ATCC 8750, x * 44000, strain JM3 | Alcaligenes faecalis |
| ? | x * 37000 | Fusarium oxysporum |
| ? | x * 40000, strain J1 | Rhodococcus rhodochrous |
| ? | x * 41000 | Rhodococcus rhodochrous |
| ? | x * 41000 + x * 72000, enzyme + groEL | Aeribacillus pallidus |
| ? | x * 41000-43000 | Acinetobacter sp. |
| ? | x * 45000, strain NCIB11216 and strain NCIB11215 | Nocardia sp. |
| ? | x * 76000 | Fusarium solani |
| dimer | 2 x 37000 | Klebsiella pneumoniae |
| monomer | 1 x * 45000, strain PA-34 | Rhodococcus rhodochrous |
| monomer | 1 x 41000, strain SI | Pseudomonas sp. |
| oligomer | x * 38000 | Comamonas testosteroni |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Rhodococcus sp. |
| 30 | - |
strain NCIB11216 and strain NCIB11215 | Nocardia sp. |
| 35 | - |
- |
Klebsiella pneumoniae |
| 35 | - |
strain PA-34 | Rhodococcus rhodochrous |
| 40 | 45 | strain ATCC 8750 | Alcaligenes faecalis |
| 40 | - |
- |
Fusarium oxysporum |
| 44 | - |
strain JM3 | Alcaligenes faecalis |
| 45 | - |
strain J1 | Rhodococcus rhodochrous |
| 50 | - |
- |
Acinetobacter sp. |
| 55 | - |
- |
Rhodococcus rhodochrous |
| 65 | - |
- |
Aeribacillus pallidus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Rhodococcus rhodochrous |
| 6 | 9 | - |
Aeribacillus pallidus |
| 6 | 11 | - |
Fusarium oxysporum |
| 7 | 9.5 | strain NCIB11215 | Nocardia sp. |
| 7.5 | - |
- |
Rhodococcus sp. |
| 7.5 | - |
strain PA-34 | Rhodococcus rhodochrous |
| 7.5 | - |
strains ATCC 8750 and JM3 | Alcaligenes faecalis |
| 7.6 | - |
strain J1 | Rhodococcus rhodochrous |
| 7.8 | 9.1 | - |
Fusarium solani |
| 8 | - |
- |
Acinetobacter sp. |
| 8 | - |
strain NCIB11216 | Nocardia sp. |
| 9.2 | - |
- |
Klebsiella pneumoniae |
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