Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.5.1 extracted from

  • Levy-Schil, S.; Soubrier, F.; Crutz-Le Coq, A.M.; Faucher, D.; Crouzet, J.; Petre, D.
    Aliphatic nitrilase from a soil-isolated Comamonas testosteroni sp.: gene cloning and overexpression, purification and primary structure (1995), Gene, 161, 15-20.
    View publication on PubMed
Search for more literature for 3.5.5.1

Application

Application Comment Organism
synthesis nitrilase products are intermediates in the synthesis of nylon Comamonas testosteroni

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Comamonas testosteroni

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acrylonitrile + H2O Comamonas testosteroni
-
 acrylic acid + NH3
-
 ?
benzonitrile + 2 H2O Comamonas testosteroni low activity benzoic acid + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Comamonas testosteroni Q59329
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, recombinant enzyme Comamonas testosteroni

Source Tissue

Source Tissue Comment Organism Textmining
additional information nitA gene Comamonas testosteroni
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
68
-
-
 Comamonas testosteroni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acrylonitrile + H2O
-
 Comamonas testosteroni acrylic acid + NH3
-
 ?
adiponitrile + H2O preferred substrate, specific for aliphatic nitriles Comamonas testosteroni adipic acid + NH3
-
 ?
adiponitrile + H2O no formation of amide Comamonas testosteroni adipic acid + NH3
-
 ?
benzonitrile + 2 H2O low activity Comamonas testosteroni benzoic acid + NH3
-
 ?
cyano-5-valeramide + H2O
-
 Comamonas testosteroni valeramide + NH3
-
 ?
cyano-5-valeric acid + H2O
-
 Comamonas testosteroni valeric acid + NH3
-
 ?
propionitrile + H2O low activity Comamonas testosteroni propionic acid + NH3
-
 ?