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Literature summary for 3.5.5.1 extracted from

  • Bhalla, T.C.; Miura, A.; Wakamoto, A.; Ohba, Y.; Furuhashi, K.
    Asymmetric hydrolysis of alpha-aminonitriles to optically active amino acids by a nitrilase of Rhodococcus rhodochrous PA-34 (1992), Appl. Microbiol. Biotechnol., 37, 184-190.
No PubMed abstract available
Search for more literature for 3.5.5.1

Activating Compound

Activating Compound Comment Organism Structure
hydroxylamine 20% activation at 1 mM Rhodococcus rhodochrous

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzoate nearly complete inhibition at 1 mM Rhodococcus rhodochrous
5,5'-dithiobis(2-nitrobenzoic acid) complete inhibition at 1 mM Rhodococcus rhodochrous
Ag+ complete inhibition at 5 mM Rhodococcus rhodochrous
Co2+ complete inhibition at 1 mM Rhodococcus rhodochrous
Hg2+ 43% inhibition at 1 mM Rhodococcus rhodochrous
iodoacetic acid slight inhibition at 1 mM Rhodococcus rhodochrous
o-phenanthroline complete inhibition at 5 mM Rhodococcus rhodochrous
Zn2+ complete inhibition at 1 mM Rhodococcus rhodochrous

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 gel filtration Rhodococcus rhodochrous

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acrylonitrile + H2O Rhodococcus rhodochrous
-
 acrylic acid + NH3
-
 ?
benzonitrile + 2 H2O Rhodococcus rhodochrous best substrate benzoic acid + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Rhodococcus rhodochrous
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography techniques Rhodococcus rhodochrous

Source Tissue

Source Tissue Comment Organism Textmining
cell culture best inducer of enzyme formation: isobutyronitrile Rhodococcus rhodochrous
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.52
-
-
 Rhodococcus rhodochrous

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminobutyronitrile + H2O
-
 Rhodococcus rhodochrous 2-aminobutyric acid + NH3
-
 ?
2-aminopropionitrile + H2O low activity Rhodococcus rhodochrous 2-aminopropionic acid + NH3
-
 ?
2-methylbutyronitrile + H2O
-
 Rhodococcus rhodochrous 2-methylbutyric acid + NH3
-
 ?
acrylonitrile + H2O
-
 Rhodococcus rhodochrous acrylic acid + NH3
-
 ?
benzonitrile + 2 H2O best substrate Rhodococcus rhodochrous benzoic acid + NH3
-
 ?
crotononitrile + H2O
-
 Rhodococcus rhodochrous NH3 + (z)-but-2-en-1-ol
-
 ?
DL-alpha-amino-4-methylthio butyronitrile + H2O
-
 Rhodococcus rhodochrous L-methionine + NH3
-
 ?
DL-alpha-amino-n-capronitrile + H2O
-
 Rhodococcus rhodochrous L-norleucine + NH3
-
 ?
DL-alpha-amino-n-valeronitrile + H2O
-
 Rhodococcus rhodochrous L-norvaline + NH3
-
 ?
DL-alpha-aminoisocapronitrile + H2O
-
 Rhodococcus rhodochrous L-leucine + NH3
-
 ?
DL-alpha-aminoisovaleronitrile + H2O
-
 Rhodococcus rhodochrous L-valine + NH3
-
 ?
DL-alpha-aminopropionitrile + H2O
-
 Rhodococcus rhodochrous D-alanine + NH3
-
 ?
lactonitrile + H2O
-
 Rhodococcus rhodochrous lactate + NH3
-
 ?
mandelonitrile + 2 H2O
-
 Rhodococcus rhodochrous mandelic acid + NH3
-
 ?
methacrylonitrile + H2O low activity Rhodococcus rhodochrous methylacrylic acid + NH3
-
 ?
n-butyronitrile + H2O
-
 Rhodococcus rhodochrous n-butyric acid + NH3
-
 ?
phenylacetonitrile + 2 H2O low activity Rhodococcus rhodochrous phenylacetate + NH3
-
 ?
phenylglycinonitrile + H2O low actiity Rhodococcus rhodochrous phenylglycine + NH3
-
 ?
propionitrile + H2O
-
 Rhodococcus rhodochrous propionic acid + NH3
-
 ?
succinonitrile + H2O low activity Rhodococcus rhodochrous succinate + NH3
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 45000, SDS-PAGE Rhodococcus rhodochrous

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Rhodococcus rhodochrous

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
 decreasing activity above Rhodococcus rhodochrous

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Rhodococcus rhodochrous