Application | Comment | Organism |
---|---|---|
synthesis | preparing carboxylic acids from readily available nitrile analogues | Rhodococcus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) | complete inhibition at 1 mM | Rhodococcus sp. | |
benzaldehyde | nearly complete inhibition at 10 mM | Rhodococcus sp. | |
benzyl bromide | strong inhibition at 10 mM | Rhodococcus sp. | |
benzylamine | 65% inhibition at 10 mM | Rhodococcus sp. | |
Hg2+ | nearly complete inactivation at 1 mM | Rhodococcus sp. | |
phenylacetonitrile | 50% inhibition at 10 mM | Rhodococcus sp. | |
phenylmethylsulfonyl fluoride | 40% inhibition at 1 mM | Rhodococcus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
3-chlorobenzonitrile | - |
Rhodococcus sp. | |
2 | - |
4-nitrobenzonitrile | - |
Rhodococcus sp. | |
2.4 | - |
3-methylbenzonitrile | - |
Rhodococcus sp. | |
2.6 | - |
3-cyanobenzonitrile | - |
Rhodococcus sp. | |
2.7 | - |
3-Methoxybenzonitrile | - |
Rhodococcus sp. | |
3.5 | - |
3-aminobenzonitrile | - |
Rhodococcus sp. | |
3.8 | - |
Benzonitrile | - |
Rhodococcus sp. | |
4.8 | - |
3-Nitrobenzonitrile | - |
Rhodococcus sp. | |
8 | - |
4-aminobenzonitrile | - |
Rhodococcus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41500 | - |
1 * 41500, SDS-PAGE | Rhodococcus sp. |
46000 | - |
gel filtration, aggregation to 560 kDa in the presence of benzonitrile | Rhodococcus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-cyanopyridine + 2 H2O | Rhodococcus sp. | - |
nicotinic acid + NH3 | - |
? | |
acrylonitrile + H2O | Rhodococcus sp. | - |
acrylic acid + NH3 | - |
? | |
benzonitrile + 2 H2O | Rhodococcus sp. | - |
benzoic acid + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus sp. | - |
strain ATCC 39484 | - |
Purification (Comment) | Organism |
---|---|
to homogeneity | Rhodococcus sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Rhodococcus sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
84 | - |
- |
Rhodococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 phenylacetonitrile + 3 H2O | - |
Rhodococcus sp. | phenylacetamide + phenylacetate + NH3 | not completely converted to acid, 2% of the product is phenylacetamide | ? | |
2-furanocarbonitrile + H2O | best substrate | Rhodococcus sp. | furan-2-carboxylic acid + NH3 | - |
? | |
3-aminobenzonitrile + H2O | - |
Rhodococcus sp. | 3-aminobenzoic acid + NH3 | - |
? | |
3-chlorobenzonitrile + H2O | - |
Rhodococcus sp. | 3-chlorobenzoic acid + NH3 | - |
? | |
3-cyanobenzonitrile + H2O | - |
Rhodococcus sp. | 3-cyanobenzoic acid + NH3 | - |
? | |
3-cyanopyridine + 2 H2O | - |
Rhodococcus sp. | nicotinic acid + NH3 | - |
? | |
3-methoxybenzonitrile + H2O | - |
Rhodococcus sp. | 3-methoxybenzoic acid + NH3 | - |
? | |
3-nitrobenzonitrile + H2O | - |
Rhodococcus sp. | 3-nitrobenzoic acid + NH3 | - |
? | |
3-toluonitrile + H2O | - |
Rhodococcus sp. | NH3 + 3-methylbenzoate | - |
? | |
4-aminobenzonitrile + H2O | - |
Rhodococcus sp. | 4-aminobenzoic acid + NH3 | - |
? | |
4-nitrobenzonitrile + H2O | - |
Rhodococcus sp. | 4-nitrobenzoic acid + NH3 | - |
? | |
acrylonitrile + H2O | - |
Rhodococcus sp. | acrylic acid + NH3 | - |
? | |
benzonitrile + 2 H2O | - |
Rhodococcus sp. | benzoic acid + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 41500, SDS-PAGE | Rhodococcus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
irreversible inactivation by preincubation above | Rhodococcus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Rhodococcus sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9.5 | active within | Rhodococcus sp. |
5.5 | 8 | stable between | Rhodococcus sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
10 | - |
phenylacetonitrile | - |
Rhodococcus sp. |