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Literature summary for 3.5.5.1 extracted from

  • Bartling, D.; Seedorf, M.; Schmidt, R.C.; Weiler, E.W.
    Molecular characterization of two cloned nitrilases from Arabidopsis thaliana: Key enzymes in biosynthesis of the plant hormone indole-3-acetic acid (1994), Proc. Natl. Acad. Sci. USA, 91, 6021-6025.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.5.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm nitrilase I Arabidopsis thaliana 5737
-
microsome nitrilase II Arabidopsis thaliana
-
-
plasma membrane nitrilase II, tightly associated Arabidopsis thaliana 5886
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
indol-3-acetonitrile + H2O Arabidopsis thaliana
-
 indol-3-acetic acid + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nitrilase II, to homogeneity, recombinant enzyme Arabidopsis thaliana

Renatured (Commentary)

Renatured (Comment) Organism
release from insoluble inclusion bodies after treatment with Triton X-114 Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
whole plant 2 forms, nitrilase I and II Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
indol-3-acetonitrile + H2O
-
 Arabidopsis thaliana indol-3-acetic acid + NH3
-
 ?

Synonyms

Synonyms Comment Organism
nirilase II
-
 Arabidopsis thaliana
nitrilase I
-
 Arabidopsis thaliana