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Literature summary for 3.5.5.1 extracted from

  • Dhillon, J.; Chhatre, S.; Shanker, R.; Shivaraman, N.
    Transformation of aliphatic and aromatic nitriles by a nitrilase from Pseudomonas sp. (1999), Can. J. Microbiol., 45, 811-815.
No PubMed abstract available
Search for more literature for 3.5.5.1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.75
-
 potassium cyanide
-
 Pseudomonas sp.
5.88
-
 acrylonitrile
-
 Pseudomonas sp.
10.28
-
 adiponitrile
-
 Pseudomonas sp.
11.26
-
 acetonitrile
-
 Pseudomonas sp.
12.27
-
 Benzonitrile
-
 Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acrylonitrile + H2O Pseudomonas sp. best substrate, no formation of amide acrylic acid + NH3
-
 ?
benzonitrile + 2 H2O Pseudomonas sp.
-
 benzoic acid + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Pseudomonas sp.

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Pseudomonas sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2
-
-
 Pseudomonas sp.
17530
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetonitrile + H2O no formation of amide Pseudomonas sp. acetic acid + NH3
-
 ?
acrylonitrile + H2O best substrate, no formation of amide Pseudomonas sp. acrylic acid + NH3
-
 ?
adiponitrile + H2O no formation of amide Pseudomonas sp. adipic acid + NH3
-
 ?
benzonitrile + 2 H2O
-
 Pseudomonas sp. benzoic acid + NH3
-
 ?
potassium cyanide + H2O
-
 Pseudomonas sp. potassium carbonate + NH3
-
 ?