Application | Comment | Organism |
---|---|---|
synthesis | the recombinant enzyme is rapidly inactivated below pH 6.0 and at temperatures above 35 °C but under optimal conditions catalysed the highly efficient transformation of AMP to IMP with minimal by-products, suggesting that it could be used for IMP production following protein engineering | Aspergillus oryzae |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta (DE3) | Aspergillus oryzae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al3+ | 1 mM, about 80% decrease in activity | Aspergillus oryzae | |
Ba2+ | 1 mM, about 20% decrease in activity | Aspergillus oryzae | |
Co2+ | 1 mM, about 15% decrease in activity | Aspergillus oryzae | |
Cu2+ | 1 mM, about 70% decrease in activity | Aspergillus oryzae | |
Fe2+ | 1 mM, about 50% decrease in activity | Aspergillus oryzae | |
Fe3+ | 1 mM, about 45% decrease in activity | Aspergillus oryzae | |
Ni2+ | 1 mM, about 20% decrease in activity | Aspergillus oryzae | |
Zn2+ | 1 mM, about 50% decrease in activity | Aspergillus oryzae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.27 | - |
AMP | pH 6.0, temperature not specified in the publication | Aspergillus oryzae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | activity is enhanced by 15% following incubation with 1 mM K+. Maximal activation (about 2fold) at 30 mM K+ | Aspergillus oryzae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75000 | - |
gel filtration | Aspergillus oryzae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
AMP + H2O | Aspergillus oryzae | - |
IMP + NH3 | - |
? | |
AMP + H2O | Aspergillus oryzae 3811 | - |
IMP + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus oryzae | - |
- |
- |
Aspergillus oryzae 3811 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Aspergillus oryzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
AMP + H2O | - |
Aspergillus oryzae | IMP + NH3 | - |
? | |
AMP + H2O | - |
Aspergillus oryzae 3811 | IMP + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 78600, SDS-PAGE | Aspergillus oryzae |
Synonyms | Comment | Organism |
---|---|---|
AmpD | - |
Aspergillus oryzae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Aspergillus oryzae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 45 | 20°C: about 40% of maximal activity, 45°C: about 70% of maximal activity | Aspergillus oryzae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 40 | very stable below 20 °C, activity decreases with increasing temperature, and less than 50% of residual activity is retained after incubation for 1 h at temperatures above 40°C | Aspergillus oryzae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Aspergillus oryzae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | pH 5.0: about 60% of maximal activity, pH 7.0: about 40% of maximal activity | Aspergillus oryzae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
after incubation at 4 °C for 1 h, less than 50 % of residual activity remains below pH 5.0 | Aspergillus oryzae |
7 | - |
after incubation at 4 °C for 1 h, less than 50 % of residual activity remains above pH 7.0 | Aspergillus oryzae |
Organism | Comment | Expression |
---|---|---|
Aspergillus oryzae | induction with 0.75 mM isopropyl beta-D-l-thiogalactopyranoside results in an enzyme activity of 1773.9 U/ml | up |