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Literature summary for 3.5.4.6 extracted from

  • Li, S.; Qian, Y.; Liang, Y.; Chen, X.; Zhao, M.; Guo, Y.; Pang, Z.
    Overproduction, purification and characterization of adenylate deaminase from Aspergillus oryzae (2016), Appl. Biochem. Biotechnol., 180, 1635-1643 .
    View publication on PubMed

Application

Application Comment Organism
synthesis the recombinant enzyme is rapidly inactivated below pH 6.0 and at temperatures above 35 °C but under optimal conditions catalysed the highly efficient transformation of AMP to IMP with minimal by-products, suggesting that it could be used for IMP production following protein engineering Aspergillus oryzae

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta (DE3) Aspergillus oryzae

Inhibitors

Inhibitors Comment Organism Structure
Al3+ 1 mM, about 80% decrease in activity Aspergillus oryzae
Ba2+ 1 mM, about 20% decrease in activity Aspergillus oryzae
Co2+ 1 mM, about 15% decrease in activity Aspergillus oryzae
Cu2+ 1 mM, about 70% decrease in activity Aspergillus oryzae
Fe2+ 1 mM, about 50% decrease in activity Aspergillus oryzae
Fe3+ 1 mM, about 45% decrease in activity Aspergillus oryzae
Ni2+ 1 mM, about 20% decrease in activity Aspergillus oryzae
Zn2+ 1 mM, about 50% decrease in activity Aspergillus oryzae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.27
-
AMP pH 6.0, temperature not specified in the publication Aspergillus oryzae

Metals/Ions

Metals/Ions Comment Organism Structure
K+ activity is enhanced by 15% following incubation with 1 mM K+. Maximal activation (about 2fold) at 30 mM K+ Aspergillus oryzae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
gel filtration Aspergillus oryzae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
AMP + H2O Aspergillus oryzae
-
IMP + NH3
-
?
AMP + H2O Aspergillus oryzae 3811
-
IMP + NH3
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
-
-
Aspergillus oryzae 3811
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Aspergillus oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
AMP + H2O
-
Aspergillus oryzae IMP + NH3
-
?
AMP + H2O
-
Aspergillus oryzae 3811 IMP + NH3
-
?

Subunits

Subunits Comment Organism
? x * 78600, SDS-PAGE Aspergillus oryzae

Synonyms

Synonyms Comment Organism
AmpD
-
Aspergillus oryzae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Aspergillus oryzae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 45 20°C: about 40% of maximal activity, 45°C: about 70% of maximal activity Aspergillus oryzae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 40 very stable below 20 °C, activity decreases with increasing temperature, and less than 50% of residual activity is retained after incubation for 1 h at temperatures above 40°C Aspergillus oryzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Aspergillus oryzae

pH Range

pH Minimum pH Maximum Comment Organism
5 7 pH 5.0: about 60% of maximal activity, pH 7.0: about 40% of maximal activity Aspergillus oryzae

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
after incubation at 4 °C for 1 h, less than 50 % of residual activity remains below pH 5.0 Aspergillus oryzae
7
-
after incubation at 4 °C for 1 h, less than 50 % of residual activity remains above pH 7.0 Aspergillus oryzae

Expression

Organism Comment Expression
Aspergillus oryzae induction with 0.75 mM isopropyl beta-D-l-thiogalactopyranoside results in an enzyme activity of 1773.9 U/ml up