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Literature summary for 3.5.4.5 extracted from

  • Sanchez-Quitian, Z.; Rodrigues-Junior, V.; Rehm, J.; Eichler, P.; Barbosa Trivella, D.; Bizarro, C.; Basso, L.; Santos, D.
    Functional and structural evidence for the catalytic role played by glutamate-47 residue in the mode of action of Mycobacterium tuberculosis cytidine deaminase (2015), RSC Adv., 5, 830-840 .
No PubMed abstract available
Search for more literature for 3.5.4.5

Crystallization (Commentary)

Crystallization (Comment) Organism
structures of apomutants E47D and E47Q Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
E47A mutant protein is insoluble Mycobacterium tuberculosis
E47D 37fold decrease in kcat value. No kcat and kcat/KM dependence on pH values ranging from 4.0 to 11 is observed Mycobacterium tuberculosis
E47H mutant protein is insoluble Mycobacterium tuberculosis
E47L complete loss of activity Mycobacterium tuberculosis
E47Q 19fold decrease in kcat value Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.004
-
 cytidine wild-type, pH 7.5, 25°C Mycobacterium tuberculosis
1.461
-
 cytidine mutant E47Q, pH 7.5, 25°C Mycobacterium tuberculosis
1.612
-
 cytidine mutant E47D, pH 7.5, 25°C Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ presence of 1 mol Zn2+ per mol of subunit Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 PAGE Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WPH3
-
-
Mycobacterium tuberculosis H37Rv P9WPH3
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.46
-
 wild-type, substrate 2'-deoxycytidine, pH 7.5, 25°C Mycobacterium tuberculosis
4.75
-
 wild-type, substrate cytidine, pH 7.5, 25°C Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-deoxycytidine + H2O
-
 Mycobacterium tuberculosis 2'-deoxyuridne + NH3
-
 ?
2'-deoxycytidine + H2O
-
 Mycobacterium tuberculosis H37Rv 2'-deoxyuridne + NH3
-
 ?
cytidine + H2O
-
 Mycobacterium tuberculosis uridine + NH3
-
 ?
cytidine + H2O
-
 Mycobacterium tuberculosis H37Rv uridine + NH3
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 13900, SDS-PAGE Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.13
-
 cytidine mutant E47D, pH 7.5, 25°C Mycobacterium tuberculosis
0.26
-
 cytidine mutant E47Q, pH 7.5, 25°C Mycobacterium tuberculosis
4.85
-
 cytidine wild-type, pH 7.5, 25°C Mycobacterium tuberculosis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.08
-
 cytidine mutant E47D, pH 7.5, 25°C Mycobacterium tuberculosis
0.178
-
 cytidine mutant E47Q, pH 7.5, 25°C Mycobacterium tuberculosis
4.83
-
 cytidine wild-type, pH 7.5, 25°C Mycobacterium tuberculosis