Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | [Bacillus] caldolyticus |
expression in Escherichia coli | Sporosarcina psychrophila |
General Stability | Organism |
---|---|
irreversible denaturation after 30 min at 72°C | Bacillus subtilis |
irreversible denaturation at 72°C after 30 min | Sporosarcina psychrophila |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
14237 | - |
4 * 14237, calculation from nucleotide sequence | [Bacillus] caldolyticus |
14600 | - |
4 * 14600, calculation from nucleotide sequence | Sporosarcina psychrophila |
15000 | - |
4 * 15000, SDS-PAGE | [Bacillus] caldolyticus |
15000 | - |
4 * 15000, SDS-PAGE | Sporosarcina psychrophila |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Sporosarcina psychrophila | Q9S3M0 | - |
- |
[Bacillus] caldolyticus | Q9R2S1 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
[Bacillus] caldolyticus |
- |
Sporosarcina psychrophila |
Renatured (Comment) | Organism |
---|---|
after partial denaturation at 72°C for 30 min and following renaturation on ice, the enzyme retains its enzymatic activity | [Bacillus] caldolyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxycytidine + H2O | - |
Bacillus subtilis | deoxyuridine + NH3 | - |
? | |
deoxycytidine + H2O | - |
[Bacillus] caldolyticus | deoxyuridine + NH3 | - |
? | |
deoxycytidine + H2O | - |
Sporosarcina psychrophila | deoxyuridine + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 15000, SDS-PAGE | [Bacillus] caldolyticus |
tetramer | 4 * 15000, SDS-PAGE | Sporosarcina psychrophila |
tetramer | 4 * 14237, calculation from nucleotide sequence | [Bacillus] caldolyticus |
tetramer | 4 * 14600, calculation from nucleotide sequence | Sporosarcina psychrophila |
Synonyms | Comment | Organism |
---|---|---|
CDABcald | - |
[Bacillus] caldolyticus |
CDABpsy | - |
Sporosarcina psychrophila |
CDABsub | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
33 | - |
- |
Sporosarcina psychrophila |
37 | - |
- |
Bacillus subtilis |
56 | 62 | - |
[Bacillus] caldolyticus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
6 | 70 | 6°C: 45% of maximal activity, 70°C: 35% of maximal activity | Sporosarcina psychrophila |
12 | 66 | 12°C: about 55% of maximal activity, 66°C: about 65% of maximal activity | Bacillus subtilis |
34 | 74 | 34°C: 45% of maximal activity, 74°C: 70% of maximal activity | [Bacillus] caldolyticus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
stable up to | [Bacillus] caldolyticus |
72 | - |
30 min, irreversible denaturation | Bacillus subtilis |
72 | - |
30 min, irreversible denaturation | Sporosarcina psychrophila |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bacillus subtilis | chromatofocusing | - |
4.2 |
Sporosarcina psychrophila | chromatofocusing | - |
4.2 |
[Bacillus] caldolyticus | chromatofocusing | - |
4.9 |