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Literature summary for 3.5.4.5 extracted from

  • Vincenzetti, S.; Cambi, A.; Neuhard, J.; Schnorr, K.; Grelloni, M.; Vita, A.
    Cloning, expression, and purification of cytidine deaminase from Arabidopsis thaliana (1999), Protein Expr. Purif., 15, 8-15.
    View publication on PubMed
Search for more literature for 3.5.4.5

Cloned(Commentary)

Cloned (Comment) Organism
cDNA coding for AT-CDA1 amplified, subcloned into pTrc99-A, induced and expressed and overproduced in Escherichia coli SO5201 Arabidopsis thaliana

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
3,4,5,6-Tetrahydrouridine
-
 Arabidopsis thaliana
3,4,5,6-Tetrahydrouridine
-
 Escherichia coli
3,4,5,6-Tetrahydrouridine
-
 Mus musculus
3-deazauridine
-
 Arabidopsis thaliana
5,6-Dihydrouridine
-
 Arabidopsis thaliana
5,6-Dihydrouridine
-
 Escherichia coli
5-fluoro-2'-deoxyuridine
-
 Arabidopsis thaliana
5-fluorouridine
-
 Arabidopsis thaliana
5-{(1E)-3-[(5-carboxypentyl)amino]-3-oxoprop-1-en-1-yl}-5,6-dihydrocytidine
-
 Arabidopsis thaliana
CMP
-
 Arabidopsis thaliana
thymine riboside
-
 Arabidopsis thaliana
thymine riboside
-
 Escherichia coli
uridine
-
 Arabidopsis thaliana
uridine
-
 Escherichia coli
UTP
-
 Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.058
-
 cytosine arabinoside recombinant AT-CDA Arabidopsis thaliana
0.06
-
 deoxycytidine
-
 Escherichia coli
0.075
-
 deoxycytidine recombinant AT-CDA Arabidopsis thaliana
0.11
-
 cytidine
-
 Escherichia coli
0.15
-
 cytidine recombinant AT-CDA Arabidopsis thaliana
0.22
-
 cytosine arabinoside
-
 Escherichia coli
0.31
-
 5-azacytidine recombinant AT-CDA Arabidopsis thaliana
0.58
-
 5-methylcytidine recombinant Arabidopsis thaliana
0.95
-
 5-methylcytidine
-
 Escherichia coli
2.27
-
 5-azacytidine
-
 Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
15000
-
 4 * 15000 Gallus gallus
32000
-
 2 * 32000, homodimer, SDS-PAGE Escherichia coli
32000
-
 2 * 32000, homodimer, SDS-PAGE Arabidopsis thaliana
32560
-
 calculated from cDNA open reading frame Arabidopsis thaliana
32580
-
 calculated from amino acid sequence Arabidopsis thaliana
63000
-
 gel filtration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cytidine + H2O Gallus gallus
-
 uridine + NH3
-
 ?
cytidine + H2O Haemophilus influenzae
-
 uridine + NH3
-
 ?
cytidine + H2O Bacillus subtilis
-
 uridine + NH3
-
 ?
cytidine + H2O Mus musculus
-
 uridine + NH3
-
 ?
cytidine + H2O Escherichia coli
-
 uridine + NH3
-
 ?
cytidine + H2O Ovis aries
-
 uridine + NH3
-
 ?
cytidine + H2O Arabidopsis thaliana
-
 uridine + NH3
-
 ?
cytidine + H2O Escherichia coli SO5201
-
 uridine + NH3
-
 ?
cytidine + H2O Mus musculus BALB/c
-
 uridine + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Bacillus subtilis
-
-
-
Escherichia coli
-
-
-
Escherichia coli SO5201
-
-
-
Gallus gallus
-
 chicken
-
Haemophilus influenzae
-
-
-
Mus musculus
-
 mouse
-
Mus musculus
-
 BALB/c
-
Mus musculus BALB/c
-
 BALB/c
-
Ovis aries
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Arabidopsis thaliana

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
74
-
-
 Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-azacytidine + H2O
-
 Escherichia coli 5-azauridine + NH3
-
 ?
5-azacytidine + H2O
-
 Arabidopsis thaliana 5-azauridine + NH3
-
 ?
5-azacytidine + H2O
-
 Escherichia coli SO5201 5-azauridine + NH3
-
 ?
5-azadeoxycytidine + H2O
-
 Arabidopsis thaliana 5-azadeoxyuridine + NH3
-
 ?
5-methylcytidine + H2O
-
 Escherichia coli 5-methyluridine + NH3
-
 ?
5-methylcytidine + H2O
-
 Arabidopsis thaliana 5-methyluridine + NH3
-
 ?
5-methylcytidine + H2O
-
 Escherichia coli SO5201 5-methyluridine + NH3
-
 ?
cytidine + H2O
-
 Gallus gallus uridine + NH3
-
 ?
cytidine + H2O
-
 Haemophilus influenzae uridine + NH3
-
 ?
cytidine + H2O
-
 Bacillus subtilis uridine + NH3
-
 ?
cytidine + H2O
-
 Mus musculus uridine + NH3
-
 ?
cytidine + H2O
-
 Escherichia coli uridine + NH3
-
 ?
cytidine + H2O
-
 Ovis aries uridine + NH3
-
 ?
cytidine + H2O
-
 Arabidopsis thaliana uridine + NH3
-
 ?
cytidine + H2O
-
 Escherichia coli SO5201 uridine + NH3
-
 ?
cytidine + H2O
-
 Mus musculus BALB/c uridine + NH3
-
 ?
cytosine arabinoside + H2O
-
 Mus musculus uridine arabinoside + NH3
-
 ?
cytosine arabinoside + H2O
-
 Escherichia coli uridine arabinoside + NH3
-
 ?
cytosine arabinoside + H2O
-
 Arabidopsis thaliana uridine arabinoside + NH3
-
 ?
cytosine arabinoside + H2O
-
 Escherichia coli SO5201 uridine arabinoside + NH3
-
 ?
cytosine arabinoside + H2O
-
 Mus musculus BALB/c uridine arabinoside + NH3
-
 ?
deoxycytidine + H2O
-
 Mus musculus deoxyuridine + NH3
-
 ?
deoxycytidine + H2O
-
 Escherichia coli deoxyuridine + NH3
-
 ?
deoxycytidine + H2O
-
 Arabidopsis thaliana deoxyuridine + NH3
-
 ?
deoxycytidine + H2O
-
 Escherichia coli SO5201 deoxyuridine + NH3
-
 ?
deoxycytidine + H2O
-
 Mus musculus BALB/c deoxyuridine + NH3
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 32000, homodimer, SDS-PAGE Escherichia coli
dimer 2 * 32000, homodimer, SDS-PAGE Arabidopsis thaliana
tetramer
-
 Bacillus subtilis
tetramer 4 * 15000 Gallus gallus

pH Range

pH Minimum pH Maximum Comment Organism
3 10.5 deoxycytidine as substrate, specific activity constant in this range, beyond these values irreversible inactivation occurs Arabidopsis thaliana

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 10.5 deoxycytidine as substrate, beyond these extremes rapid inactivation Arabidopsis thaliana