Cloned (Comment) | Organism |
---|---|
cDNA, PCR, vector pTrc99A, cloned and expressed in Escherichia coli DH5alpha | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
- |
Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3,4,5,6-Tetrahydrouridine | recombinant CDA | Homo sapiens | |
3-deazauridine | recombinant enzyme | Homo sapiens | |
5-fluoro-2'-deoxyuridine | recombinant enzyme | Homo sapiens | |
5-{(1E)-3-[(5-carboxypentyl)amino]-3-oxoprop-1-en-1-yl}-5,6-dihydrocytidine | recombinant enzyme | Homo sapiens | |
CMP | recombinant CDA | Homo sapiens | |
thymine riboside | recombinant CDA | Homo sapiens | |
uridine | - |
Escherichia coli | |
uridine | recombinant enzyme | Homo sapiens | |
UTP | recombinant CDA | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.039 | - |
cytidine | recombinant CDA | Homo sapiens | |
0.039 | - |
deoxycytidine | recombinant CDA | Homo sapiens | |
0.04 | - |
5-methylcytidine | recombinant CDA | Homo sapiens | |
0.1 | - |
5-iododeoxycytidine | recombinant CDA | Homo sapiens | |
0.11 | - |
cytosine arabinoside | recombinant CDA | Homo sapiens | |
0.28 | - |
5-azacytidine | recombinant CDA | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
15000 | - |
4 * 15000, SDS-PAGE, purified enzyme, homotetramer | Homo sapiens |
16000 | - |
estimated from cDNA open reading frame coding for human cytidine deaminase | Homo sapiens |
16170 | - |
deduced from cDNA nucleotide sequence | Homo sapiens |
32000 | - |
2 * 32000, homodimer, SDS-PAGE | Escherichia coli |
57000 | - |
gel filtration | Escherichia coli |
57000 | - |
gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cytidine + H2O | Bacillus subtilis | - |
uridine + NH3 | - |
? | |
cytidine + H2O | Escherichia coli | - |
uridine + NH3 | - |
? | |
cytidine + H2O | Homo sapiens | - |
uridine + NH3 | - |
? | |
cytidine + H2O | Escherichia coli DH5-alpha | - |
uridine + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Escherichia coli | - |
strain DH5alpha | - |
Escherichia coli DH5-alpha | - |
strain DH5alpha | - |
Homo sapiens | - |
human | - |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
granulocyte | - |
Homo sapiens | - |
leukocyte | - |
Homo sapiens | - |
liver | - |
Homo sapiens | - |
lymphocyte | - |
Homo sapiens | - |
placenta | - |
Homo sapiens | - |
spleen | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
105 | - |
- |
Homo sapiens |
Storage Stability | Organism |
---|---|
-20°C, 20 mM Tris-HCl, pH 7.5, 1 mM DDT, 1 mM EDTA, 20% glycerol, enzyme retains full activity for at least 1 month, 80% of activity lost in absence of glycerol | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-beta-D-arabinofuranosylcytosine + H2O | - |
Escherichia coli | ? | - |
? | |
1-beta-D-arabinofuranosylcytosine + H2O | - |
Homo sapiens | ? | - |
? | |
1-beta-D-arabinofuranosylcytosine + H2O | - |
Escherichia coli DH5-alpha | ? | - |
? | |
5-azacytidine + H2O | - |
Homo sapiens | 5-azauridine + NH3 | - |
? | |
5-azadeoxycytidine + H2O | - |
Homo sapiens | 5-azadeoxyuridine + NH3 | - |
? | |
5-methylcytidine + H2O | - |
Homo sapiens | 5-methyluridine + NH3 | - |
? | |
cytidine + H2O | - |
Bacillus subtilis | uridine + NH3 | - |
? | |
cytidine + H2O | - |
Escherichia coli | uridine + NH3 | - |
? | |
cytidine + H2O | - |
Homo sapiens | uridine + NH3 | - |
? | |
cytidine + H2O | - |
Escherichia coli DH5-alpha | uridine + NH3 | - |
? | |
deoxycytidine + H2O | - |
Escherichia coli | deoxyuridine + NH3 | - |
? | |
deoxycytidine + H2O | - |
Homo sapiens | deoxyuridine + NH3 | - |
? | |
deoxycytidine + H2O | - |
Escherichia coli DH5-alpha | deoxyuridine + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 32000, homodimer, SDS-PAGE | Escherichia coli |
tetramer | - |
Bacillus subtilis |
tetramer | 4 * 16000, amino acid sequence | Homo sapiens |
tetramer | 4 * 15000, SDS-PAGE, purified enzyme, homotetramer | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 10.5 | deoxycytidine as substrate, beyond these values irreversible inactivation occurs | Homo sapiens |