Literature summary for 3.5.4.5 extracted from

Vita, A.; Amici, A.; Cacciamani, T.; Lanciotti, M.; Magni, G.
Cytidine deaminase from Escherichia coli B. Purification and enzymatic and molecular properties (1985), Biochemistry, 24, 6020-6024.

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Inhibitors

Inhibitors	Comment	Organism	Structure
1-methyladenosine	competitive inhibition	Escherichia coli
5,6-Dihydrouridine	-	Escherichia coli
deoxyadenosine	competitive inhibition	Escherichia coli
deoxyuridine	competitive inhibition	Escherichia coli
dihydrothymidine	competitive inhibition	Escherichia coli
NH3	product inhibition	Escherichia coli
thymidine	competitive inhibition	Escherichia coli
thymine riboside	competitive inhibition	Escherichia coli
uridine	product inhibition	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.09	-	deoxycytidine	-	Escherichia coli
0.18	-	cytidine	-	Escherichia coli
1.25	-	5-methylcytidine	-	Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
33000	-	2 * 33000, SDS-PAGE	Escherichia coli
56000	-	gel filtration, Sephacryl S-300	Escherichia coli
66000	-	gel filtration, SDS-PAGE	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
cytidine + H2O	Escherichia coli	-	uridine + NH3	-	?
cytidine + H2O	Escherichia coli B / ATCC 11303	-	uridine + NH3	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-
Escherichia coli B / ATCC 11303	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Escherichia coli

Storage Stability

Storage Stability	Organism
-20°C, 50 mM phosphate buffer pH7.5 after 1 month pure enzyme retains 70% original activity	Escherichia coli
-20°C, 50 mM Tris-HCl, pH7.5, after 1 month pure enzyme retains 80% original activity	Escherichia coli
4°C, 50 mM Tris-HCl, pH 7.5, pure enzyme retains 63% original activity after 2 weeks	Escherichia coli
4°C,50 mM phosphate buffer pH 7.5, pure enzyme retains 13% original activity after 2 weeks	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5-methylcytidine + H2O	-	Escherichia coli	5-methyluridine + NH3	-	?
5-methylcytidine + H2O	-	Escherichia coli B / ATCC 11303	5-methyluridine + NH3	-	?
cytidine + H2O	-	Escherichia coli	uridine + NH3	-	?
cytidine + H2O	-	Escherichia coli B / ATCC 11303	uridine + NH3	-	?
deoxycytidine + H2O	-	Escherichia coli	deoxyuridine + NH3	-	?
deoxycytidine + H2O	-	Escherichia coli B / ATCC 11303	deoxyuridine + NH3	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 33000, SDS-PAGE	Escherichia coli

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Release 2023.1 (January 2023)