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Literature summary for 3.5.4.5 extracted from

  • Ipata, P.L.; Cercignani, G.
    Cytosine and cytidine deaminase from yeast (1978), Methods Enzymol., 51, 394-401.
    View publication on PubMed
Search for more literature for 3.5.4.5

Inhibitors

Inhibitors Comment Organism Structure
CDP
-
 Saccharomyces cerevisiae
CMP 0.300 mM 50% inhibition, 0.750 mM 90% inhibition Saccharomyces cerevisiae
CTP
-
 Saccharomyces cerevisiae
cytidine strong substrate inhibition above 0.5 mM Saccharomyces cerevisiae
dTMP 0.400 mM 50% inhibition Saccharomyces cerevisiae
dTTP
-
 Mus musculus
dTTP 0.400 mM 50% inhibition Saccharomyces cerevisiae
GTP 0.750 mM 85% inhibition Saccharomyces cerevisiae
p-mercuribenzoate highly sensitive Mus musculus
p-mercuribenzoate highly sensitive Saccharomyces cerevisiae
UTP
-
 Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.091
-
 2-deoxycytidine
-
 Saccharomyces cerevisiae
0.25
-
 cytidine
-
 Escherichia coli
0.25
-
 cytidine
-
 Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
 gel filtration Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cytidine + H2O Mus musculus
-
 uridine + NH3
-
 ?
cytidine + H2O Escherichia coli
-
 uridine + NH3
-
 ?
cytidine + H2O Saccharomyces cerevisiae
-
 uridine + NH3
-
 ?
cytidine + H2O Mus musculus BALB/c
-
 uridine + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Mus musculus
-
 mouse
-
Mus musculus
-
 BALB/c
-
Mus musculus BALB/c
-
 BALB/c
-
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.128
-
-
 Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
-20°C, shows no appreciable loss of activity within 1 month Saccharomyces cerevisiae
4°C, stable for some hours at pH 5.5-9.5 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-deoxycytidine + H2O
-
 Mus musculus 2'-deoxyuridine + NH3
-
 ?
2'-deoxycytidine + H2O
-
 Escherichia coli 2'-deoxyuridine + NH3
-
 ?
2'-deoxycytidine + H2O
-
 Saccharomyces cerevisiae 2'-deoxyuridine + NH3
-
 ?
2'-deoxycytidine + H2O
-
 Mus musculus BALB/c 2'-deoxyuridine + NH3
-
 ?
cytidine + H2O
-
 Mus musculus uridine + NH3
-
 ?
cytidine + H2O
-
 Escherichia coli uridine + NH3
-
 ?
cytidine + H2O
-
 Saccharomyces cerevisiae uridine + NH3
-
 ?
cytidine + H2O
-
 Mus musculus BALB/c uridine + NH3
-
 ?

Synonyms

Synonyms Comment Organism
yeast cytidine deaminase
-
 Saccharomyces cerevisiae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 62 heat treatment for 5 min at 50-60°C completely abolishes CMP and substrate inhibition Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
 Mus musculus
7.2
-
-
 Saccharomyces cerevisiae

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 9.5 stable for some hours at 4°C, rapid inactivation and precipitation of the enzyme occurs at pH lower 5 Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.3
-
 CMP
-
 Saccharomyces cerevisiae
0.4
-
 dTMP
-
 Saccharomyces cerevisiae