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Literature summary for 3.5.4.4 extracted from

  • Jaruwat, A.; Riangrungroj, P.; Ubonprasert, S.; Sae-Ueng, U.; Kuaprasert, B.; Yuthavong, Y.; Leartsakulpanich, U.; Chitnumsub, P.
    Crystal structure of Plasmodium falciparum adenosine deaminase reveals a novel binding pocket for inosine (2019), Arch. Biochem. Biophys., 667, 6-13 .
    View publication on PubMed
Search for more literature for 3.5.4.4

Cloned(Commentary)

Cloned (Comment) Organism
-
 Homo sapiens
expressed in Escherichia coli BL21(DE3) Plasmodium falciparum

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure of a surface engineered adenosine deaminase at a resolution of 2.48 A Plasmodium falciparum

Protein Variants

Protein Variants Comment Organism
C27Q/L227I the mutation promotes crystallization via crystal packing and to prevents intermolecular disulfide bond formation. The surface engineered enzyme is kinetically comparable to the wild type enzyme Plasmodium falciparum
D176A mutation reduces adenosine and 5'-methylthioadenosine substrate affnity Plasmodium falciparum
D176M mutation reduces adenosine and 5'-methylthioadenosine substrate affnity Plasmodium falciparum
F136L mutant enzyme shows increased Km (more than 10fold) for adenosine and 5'-methylthioinosine Plasmodium falciparum
M155D mutation reduces kcat Homo sapiens
T174A mutation affects 5'-methylthioadenosine binding affinity Plasmodium falciparum
T174I mutation affects 5'-methylthioadenosine binding affinity Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00943
-
 adenosine pH 8.0, temperature not specified in the publication, mutant enzyme T174I Plasmodium falciparum
0.01728
-
 adenosine pH 8.0, temperature not specified in the publication, mutant enzyme, wild-type enzyme (refolded) Homo sapiens
0.01784
-
 adenosine pH 8.0, temperature not specified in the publication, wild-type enzyme Plasmodium falciparum
0.02064
-
 adenosine pH 8.0, temperature not specified in the publication, wild-type enzyme Homo sapiens
0.02113
-
 adenosine pH 8.0, temperature not specified in the publication, mutant enzyme L179H Plasmodium falciparum
0.02344
-
 adenosine pH 8.0, temperature not specified in the publication, mutant enzyme C27Q/L227I Plasmodium falciparum
0.0306
-
 5'-methylthioadenosine pH 8.0, temperature not specified in the publication, wild-type enzyme Plasmodium falciparum
0.03874
-
 adenosine pH 8.0, temperature not specified in the publication, mutant enzyme M155A Homo sapiens
0.04203
-
 adenosine pH 8.0, temperature not specified in the publication, mutant enzyme T174A Plasmodium falciparum
0.04461
-
 5'-methylthioadenosine pH 8.0, temperature not specified in the publication, mutant enzyme C27Q/L227I Plasmodium falciparum
0.05256
-
 5'-methylthioadenosine pH 8.0, temperature not specified in the publication, mutant enzyme L179H Plasmodium falciparum
0.05641
-
 adenosine pH 8.0, temperature not specified in the publication, mutant enzyme M155A (refolded) Homo sapiens
0.12628
-
 adenosine pH 8.0, temperature not specified in the publication, mutant enzyme D176A Plasmodium falciparum
0.15897
-
 adenosine pH 8.0, temperature not specified in the publication, mutant enzyme D176A/L179A Plasmodium falciparum
0.20034
-
 5'-methylthioadenosine pH 8.0, temperature not specified in the publication, mutant enzyme T174A Plasmodium falciparum
0.22422
-
 5'-methylthioadenosine pH 8.0, temperature not specified in the publication, mutant enzyme D176A/L179A Plasmodium falciparum
0.25326
-
 5'-methylthioadenosine pH 8.0, temperature not specified in the publication, mutant enzyme D176M Plasmodium falciparum
0.26747
-
 5'-methylthioadenosine pH 8.0, temperature not specified in the publication, mutant enzyme T174I Plasmodium falciparum
0.28928
-
 5'-methylthioadenosine pH 8.0, temperature not specified in the publication, mutant enzyme D176A Plasmodium falciparum
0.29961
-
 adenosine pH 8.0, temperature not specified in the publication, mutant enzyme D176M Plasmodium falciparum
0.32206
-
 adenosine pH 8.0, temperature not specified in the publication, mutant enzyme F136L Plasmodium falciparum
0.37605
-
 5'-methylthioadenosine pH 8.0, temperature not specified in the publication, mutant enzyme F136L Plasmodium falciparum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
adenosine + H2O Homo sapiens
-
 inosine + NH3
-
 ir
adenosine + H2O Plasmodium falciparum
-
 inosine + NH3
-
 ir

Organism

Organism UniProt Comment Textmining
Homo sapiens P00813
-
-
Plasmodium falciparum Q8IJA9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens
-
 Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-methylthioadenosine + H2O
-
 Plasmodium falciparum 5'-methylthioinosine + NH3
-
 ir
adenosine + H2O
-
 Homo sapiens inosine + NH3
-
 ir
adenosine + H2O
-
 Plasmodium falciparum inosine + NH3
-
 ir
adenosine + H2O the enzyme shows no activity with 5'-methylthioinosine Homo sapiens inosine + NH3
-
 ir

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.31
-
 adenosine pH 8.0, temperature not specified in the publication, mutant enzyme D176A/L179A Plasmodium falciparum
0.33
-
 5'-methylthioadenosine pH 8.0, temperature not specified in the publication, mutant enzyme T174I Plasmodium falciparum
0.69
-
 5'-methylthioadenosine pH 8.0, temperature not specified in the publication, mutant enzyme F136L Plasmodium falciparum
0.97
-
 adenosine pH 8.0, temperature not specified in the publication, mutant enzyme D176M Plasmodium falciparum
1.31
-
 adenosine pH 8.0, temperature not specified in the publication, mutant enzyme F136L Plasmodium falciparum
1.75
-
 5'-methylthioadenosine pH 8.0, temperature not specified in the publication, mutant enzyme D176A/L179A Plasmodium falciparum
1.78
-
 5'-methylthioadenosine pH 8.0, temperature not specified in the publication, mutant enzyme D176M Plasmodium falciparum
2.42
-
 adenosine pH 8.0, temperature not specified in the publication, mutant enzyme T174I Plasmodium falciparum
4.1
-
 adenosine pH 8.0, temperature not specified in the publication, wild-type enzyme Plasmodium falciparum
4.41
-
 adenosine pH 8.0, temperature not specified in the publication, mutant enzyme L179H Plasmodium falciparum
4.48
-
 5'-methylthioadenosine pH 8.0, temperature not specified in the publication, mutant enzyme T174A Plasmodium falciparum
5.35
-
 adenosine pH 8.0, temperature not specified in the publication, mutant enzyme M155A (refolded) Homo sapiens
5.49
-
 5'-methylthioadenosine pH 8.0, temperature not specified in the publication, mutant enzyme D176A Plasmodium falciparum
5.59
-
 adenosine pH 8.0, temperature not specified in the publication, mutant enzyme D176A Plasmodium falciparum
6.16
-
 adenosine pH 8.0, temperature not specified in the publication, mutant enzyme C27Q/L227I Plasmodium falciparum
7.45
-
 adenosine pH 8.0, temperature not specified in the publication, mutant enzyme T174A Plasmodium falciparum
12.17
-
 5'-methylthioadenosine pH 8.0, temperature not specified in the publication, mutant enzyme C27Q/L227I Plasmodium falciparum
13.92
-
 5'-methylthioadenosine pH 8.0, temperature not specified in the publication, wild-type enzyme Plasmodium falciparum
14.07
-
 5'-methylthioadenosine pH 8.0, temperature not specified in the publication, mutant enzyme L179H Plasmodium falciparum
28.69
-
 adenosine pH 8.0, temperature not specified in the publication, mutant enzyme M155A Homo sapiens
40.71
-
 adenosine pH 8.0, temperature not specified in the publication, mutant enzyme, wild-type enzyme (refolded) Homo sapiens
80.04
-
 adenosine pH 8.0, temperature not specified in the publication, wild-type enzyme Homo sapiens

General Information

General Information Comment Organism
metabolism Plasmodium falciparum, a malarial pathogen, can only synthesize purine nucleotides employing a salvage pathway because it lacks de novo biosynthesis. Adenosine deaminase, one of the three purine salvage enzymes Plasmodium falciparum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1
-
 5'-methylthioadenosine pH 8.0, temperature not specified in the publication, mutant enzyme T174I Plasmodium falciparum
2
-
 adenosine pH 8.0, temperature not specified in the publication, mutant enzyme D176A/L179A Plasmodium falciparum
2
-
 5'-methylthioadenosine pH 8.0, temperature not specified in the publication, mutant enzyme F136L Plasmodium falciparum
3
-
 adenosine pH 8.0, temperature not specified in the publication, mutant enzyme D176M Plasmodium falciparum
4
-
 adenosine pH 8.0, temperature not specified in the publication, mutant enzyme F136L Plasmodium falciparum
7
-
 5'-methylthioadenosine pH 8.0, temperature not specified in the publication, mutant enzyme D176M Plasmodium falciparum
8
-
 5'-methylthioadenosine pH 8.0, temperature not specified in the publication, mutant enzyme D176A/L179A Plasmodium falciparum
20
-
 5'-methylthioadenosine pH 8.0, temperature not specified in the publication, mutant enzyme D176A Plasmodium falciparum
20
-
 5'-methylthioadenosine pH 8.0, temperature not specified in the publication, mutant enzyme T174A Plasmodium falciparum
40
-
 adenosine pH 8.0, temperature not specified in the publication, mutant enzyme D176A Plasmodium falciparum
90
-
 adenosine pH 8.0, temperature not specified in the publication, mutant enzyme M155A (refolded) Homo sapiens
180
-
 adenosine pH 8.0, temperature not specified in the publication, mutant enzyme T174A Plasmodium falciparum
210
-
 adenosine pH 8.0, temperature not specified in the publication, mutant enzyme L179H Plasmodium falciparum
230
-
 adenosine pH 8.0, temperature not specified in the publication, wild-type enzyme Plasmodium falciparum
260
-
 adenosine pH 8.0, temperature not specified in the publication, mutant enzyme C27Q/L227I Plasmodium falciparum
260
-
 adenosine pH 8.0, temperature not specified in the publication, mutant enzyme T174I Plasmodium falciparum
270
-
 5'-methylthioadenosine pH 8.0, temperature not specified in the publication, mutant enzyme C27Q/L227I Plasmodium falciparum
270
-
 5'-methylthioadenosine pH 8.0, temperature not specified in the publication, mutant enzyme L179H Plasmodium falciparum
450
-
 5'-methylthioadenosine pH 8.0, temperature not specified in the publication, wild-type enzyme Plasmodium falciparum
740
-
 adenosine pH 8.0, temperature not specified in the publication, mutant enzyme M155A Homo sapiens
2360
-
 adenosine pH 8.0, temperature not specified in the publication, mutant enzyme, wild-type enzyme (refolded) Homo sapiens
3880
-
 adenosine pH 8.0, temperature not specified in the publication, wild-type enzyme Homo sapiens