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Literature summary for 3.5.4.4 extracted from

  • Qi, Y.; Li, Y.; Bao, J.J.
    Development of a capillary electrophoresis method for analyzing adenosine deaminase and purine nucleoside phosphorylase and its application in inhibitor screening (2016), Anal. Biochem., 506, 31-44 .
    View publication on PubMed

Application

Application Comment Organism
analysis a capillary electrophoresis method for simultaneous analysis of adenosine deaminase in red blood cells is developed. The method is also successfully applied in the inhibitor screening from traditional Chinese medicines Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information strong inhibition by the traditional Chinese medicines Rhizoma Chuanxiong and Angelica sinensis Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.22
-
adenosine pH 7.4, 37°C Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
adenosine + H2O Homo sapiens
-
inosine + NH3
-
ir
adenosine + H2O Mus musculus
-
inosine + NH3
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mus musculus P03958
-
-

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
Homo sapiens
-
erythrocyte apparent activity difference between healthy and hepatoma mice Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenosine + H2O
-
Homo sapiens inosine + NH3
-
ir
adenosine + H2O
-
Mus musculus inosine + NH3
-
?

Synonyms

Synonyms Comment Organism
ADA
-
Homo sapiens
ADA
-
Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8 pH 6.5: about 85% of maximal activity, pH 8.0: about 70% of maximal activity Homo sapiens