Inhibitors | Comment | Organism | Structure |
---|---|---|---|
erythro-9-(2-hydroxy-3-nonyl)adenine | EHNA 0.1mM | Danio rerio | |
Zn2+ | decrease of 31.3% on adenosine deamination in membranes is observed in the presence of 5 mM Zn2+ | Danio rerio |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
adenosine | membrane-bound ADA | Danio rerio | |
0.22 | - |
adenosine | soluble fraction | Danio rerio |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Danio rerio | 16020 | - |
soluble | - |
Danio rerio | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no effect on activity of Ca2+, Mg2+ | Danio rerio |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenosine + H2O | Danio rerio | - |
inosine + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Danio rerio | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Danio rerio | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
relative substrate specificity in nmol NH3/min/mg of protein, adenosine 11.2, 2-deoxyadenosine 23.6, guanosine 1.8, 2-deoxyguanosine 2.0 for the soluble fraction, adenosine 20.9, 2-deoxyadenosine 5.4, guanosine 1.7, 2-deoxyguanosine 1.8 for the membrane-bound adenosine deaminase | Danio rerio |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenosine + H2O | - |
Danio rerio | inosine + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADA | - |
Danio rerio |
adenosine deaminase | - |
Danio rerio |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
the temperature of 37°C is chosen for assays in order to lessen the risk of protein denaturation in both cellular fractions | Danio rerio |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 60 | - |
Danio rerio |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
membrane-bound ADA | Danio rerio |
6 | 7 | in soluble fraction | Danio rerio |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 9 | - |
Danio rerio |