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Literature summary for 3.5.4.4 extracted from

  • Sharoyan, S.; Antonyan, A.; Mardanyan, S.; Lupidi, G.; Cristalli, G.
    Influence of dipeptidyl peptidase IV on enzymatic properties of adenosine deaminase (2006), Acta Biochim. Pol., 53, 539-546.
    View publication on PubMed
Search for more literature for 3.5.4.4

Inhibitors

Inhibitors Comment Organism Structure
1-deaza-erythro-9-(2-hydroxy-3-nonyl)adenine i.e. 1-dEHNA Bos taurus
1-deaza-erythro-9-(2-hydroxy-3-nonyl)adenine i.e. 1-dEHNA Homo sapiens
1-deazaadenosine
-
 Bos taurus
1-deazaadenosine
-
 Homo sapiens
3-deaza-erythro-9-(2-hydroxy-3-nonyl)adenine i.e. 3-dEHNA Bos taurus
3-deaza-erythro-9-(2-hydroxy-3-nonyl)adenine i.e. 3-dEHNA Homo sapiens
3-deazaadenosine
-
 Bos taurus
3-deazaadenosine
-
 Homo sapiens
erythro-9-(2-hydroxy-3-nonyl)adenine i.e. EHNA Bos taurus
erythro-9-(2-hydroxy-3-nonyl)adenine i.e. EHNA Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics of the large and the small isozyme Homo sapiens
additional information
-
 additional information kinetics of the large and the small isozyme Bos taurus
0.039
-
 2'-deoxyadenosine kidney cortex large isozyme Bos taurus
0.04
-
 2'-deoxyadenosine pleural fluid large isozyme Homo sapiens
0.042
-
 2'-deoxyadenosine blood serum large isozyme Homo sapiens
0.053
-
 adenosine blood serum large isozyme Homo sapiens
0.056
-
 adenosine kidney cortex large isozyme Bos taurus
0.07
-
 adenosine pleural fluid large isozyme Homo sapiens
0.093
-
 adenosine spleen small isozyme Bos taurus
0.129
-
 adenosine lung small isozyme Bos taurus
0.135
-
 2'-deoxyadenosine spleen small isozyme Bos taurus
0.189
-
 2'-deoxyadenosine lung small isozyme Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular LADA Homo sapiens
-
-
membrane large isozyme LADA Homo sapiens 16020
-
membrane large isozyme LADA Bos taurus 16020
-
soluble small subunit SADA Homo sapiens
-
-
soluble small subunit SADA Bos taurus
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2'-deoxyadenosine + H2O Homo sapiens
-
 2'-deoxyinosine + NH3
-
 ir
2'-deoxyadenosine + H2O Bos taurus
-
 2'-deoxyinosine + NH3
-
 ir
adenosine + H2O Homo sapiens
-
 inosine + NH3
-
 ir
adenosine + H2O Bos taurus
-
 inosine + NH3
-
 ir
additional information Bos taurus the enzyme is bound and complexed by the cell membraneous dipeptidyl peptidase IV, which is identical to the activated immune cell antigen CD26, playing a role in the immune system, overview ?
-
 ?
additional information Homo sapiens the large isozyme is bound and complexed by the cell membraneous dipeptidyl peptidase IV, which is identical to the activated immune cell antigen CD26, playing a role in the immune system, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
 small and large isozymes SADA and LADA
-
Homo sapiens
-
 small and large isozymes SADA and LADA
-

Purification (Commentary)

Purification (Comment) Organism
purification of the soluble large isozyme complexed with dipeptidyl peptidase IV from blood serum and pleural fluid by anion exchange chromatography, gel filtration, and affinity chromatography steps to about 90% purity Homo sapiens
small isozyme from lung and spleen to homogeneity, purification of the soluble large isozyme complexed with dipeptidyl peptidase IV from kidney cortex by anion exchange chromatography, gel filtration, and affinity chromatography steps to about 90% purity Bos taurus

Reaction

Reaction Comment Organism Reaction ID
adenosine + H2O = inosine + NH3 reaction mechanism, structure-function relationship of ADA and dipeptidyl peptidase IV Homo sapiens
a
adenosine + H2O = inosine + NH3 reaction mechanism, structure-function relationship of ADA and dipeptidyl peptidase IV Bos taurus
a

Source Tissue

Source Tissue Comment Organism Textmining
blood LADA Homo sapiens
-
kidney cortex Bos taurus
-
lung SADA Bos taurus
-
pleural fluid LADA Homo sapiens
-
serum LADA Homo sapiens
-
spleen SADA Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.45
-
 purified large isozyme from blood serum Homo sapiens
0.65
-
 purified large isozyme from pleural fluid Homo sapiens
5.4
-
 purified small isozyme from kidney cortex Bos taurus
350
-
 purified small isozyme from lung and spleen Bos taurus

Storage Stability

Storage Stability Organism
-20°C, purified large subunit, 2 months without loss of activity Homo sapiens
-20°C, purified large subunit, 2 months without loss of activity Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-deoxyadenosine + H2O
-
 Homo sapiens 2'-deoxyinosine + NH3
-
 ir
2'-deoxyadenosine + H2O
-
 Bos taurus 2'-deoxyinosine + NH3
-
 ir
adenosine + H2O
-
 Homo sapiens inosine + NH3
-
 ir
adenosine + H2O
-
 Bos taurus inosine + NH3
-
 ir
additional information the enzyme is bound and complexed by the cell membraneous dipeptidyl peptidase IV, which is identical to the activated immune cell antigen CD26, playing a role in the immune system, overview Bos taurus ?
-
 ?
additional information the large isozyme is bound and complexed by the cell membraneous dipeptidyl peptidase IV, which is identical to the activated immune cell antigen CD26, playing a role in the immune system, overview Homo sapiens ?
-
 ?
additional information substrate specificities of the large and the small isozyme, overview Homo sapiens ?
-
 ?
additional information substrate specificities of the large and the small isozyme, overview Bos taurus ?
-
 ?

Subunits

Subunits Comment Organism
More the enzyme exists as large and small isozymes, SADA and LADA, the latter is formed by the catalytic subunit monomer, the first by SADA complexed with the ADA complexing protein, i.e. the dipeptidyl peptidase IV Homo sapiens
More the enzyme exists as large and small isozymes, SADA and LADA, the latter is formed by the catalytic subunit monomer, the first by SADA complexed with the ADA complexing protein, i.e. the dipeptidyl peptidase IV Bos taurus

Synonyms

Synonyms Comment Organism
ADA
-
 Homo sapiens
ADA
-
 Bos taurus
LADA
-
 Homo sapiens
SADA
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7.6 large isozyme from kidney cortex Bos taurus
6.5 7.8 large isozyme from blood serum Homo sapiens
6.5 8.1 small isozyme from lung Bos taurus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information inhibition kinetics of the large and the small isozyme Bos taurus
additional information
-
 additional information inhibition kinetics of the large isozyme Homo sapiens
0.000021
-
 erythro-9-(2-hydroxy-3-nonyl)adenine kidney cortex large isozyme Bos taurus
0.000025
-
 3-deaza-erythro-9-(2-hydroxy-3-nonyl)adenine blood serum large isozyme Homo sapiens
0.00003
-
 erythro-9-(2-hydroxy-3-nonyl)adenine pleural fluid large isozyme Homo sapiens
0.000036
-
 3-deaza-erythro-9-(2-hydroxy-3-nonyl)adenine pleural fluid large isozyme Homo sapiens
0.00004
-
 erythro-9-(2-hydroxy-3-nonyl)adenine lung small isozyme Bos taurus
0.00005
-
 3-deaza-erythro-9-(2-hydroxy-3-nonyl)adenine kidney cortex large isozyme Bos taurus
0.000057
-
 erythro-9-(2-hydroxy-3-nonyl)adenine spleen small isozyme Bos taurus
0.00006
-
 erythro-9-(2-hydroxy-3-nonyl)adenine blood serum large isozyme Homo sapiens
0.00011
-
 3-deaza-erythro-9-(2-hydroxy-3-nonyl)adenine spleen small isozyme Bos taurus
0.00012
-
 3-deaza-erythro-9-(2-hydroxy-3-nonyl)adenine lung small isozyme Bos taurus
0.0004
-
 1-deaza-erythro-9-(2-hydroxy-3-nonyl)adenine kidney cortex large isozyme Bos taurus
0.00045
-
 1-deaza-erythro-9-(2-hydroxy-3-nonyl)adenine pleural fluid large isozyme Homo sapiens
0.0006
-
 1-deaza-erythro-9-(2-hydroxy-3-nonyl)adenine blood serum large isozyme Homo sapiens
0.0011
-
 1-deaza-erythro-9-(2-hydroxy-3-nonyl)adenine spleen small isozyme Bos taurus
0.0012
-
 1-deaza-erythro-9-(2-hydroxy-3-nonyl)adenine lung small isozyme Bos taurus
0.0016
-
 1-deazaadenosine kidney cortex large isozyme Bos taurus
0.0025
-
 1-deazaadenosine pleural fluid large isozyme Homo sapiens
0.0029
-
 1-deazaadenosine blood serum large isozyme Homo sapiens
0.0029
-
 1-deazaadenosine lung small isozyme Bos taurus
0.0057
-
 1-deazaadenosine spleen small isozyme Bos taurus
0.52
-
 3-deazaadenosine blood serum large isozyme Homo sapiens
0.69
-
 3-deazaadenosine kidney cortex large isozyme Bos taurus
0.81
-
 3-deazaadenosine lung small isozyme Bos taurus
1
-
 3-deazaadenosine above, pleural fluid large isozyme Homo sapiens
1
-
 3-deazaadenosine above, spleen small isozyme Bos taurus