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Literature summary for 3.5.4.4 extracted from

  • Jun, H.K.; Kim, T.S.; Sakai, T.
    Purification and characterization of extracellular adenosine deaminase from a Streptomyces sp. (1991), J. Ferment. Bioeng., 71, 6-11.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
Streptomyces sp.
Hg2+
-
Streptomyces sp.
o-phenanthroline
-
Streptomyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17
-
AMP
-
Streptomyces sp.
0.22
-
2'-deoxyadenosine
-
Streptomyces sp.
0.29
-
2',3'-isopropylidene adenosine
-
Streptomyces sp.
0.5
-
cAMP
-
Streptomyces sp.
0.67
-
adenosine
-
Streptomyces sp.
2
-
Adenosine-3'-monophosphate
-
Streptomyces sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Streptomyces sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe3+
-
Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
SDS-PAGE Streptomyces sp.
90000
-
gel filtration Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
J-845S
-
Streptomyces sp. J-845S
-
J-845S
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
211
-
-
Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2',3'-isopropylideneadenosine + H2O
-
Streptomyces sp. 2',3'-isopropylideneinosine + NH3
-
?
2',3'-isopropylideneadenosine + H2O
-
Streptomyces sp. J-845S 2',3'-isopropylideneinosine + NH3
-
?
2'-deoxyadenosine + H2O
-
Streptomyces sp. 2'-deoxyinosine + NH3
-
?
2'-deoxyadenosine + H2O
-
Streptomyces sp. J-845S 2'-deoxyinosine + NH3
-
?
5'-AMP + H2O
-
Streptomyces sp. 5'-IMP + NH3
-
?
5'-AMP + H2O
-
Streptomyces sp. J-845S 5'-IMP + NH3
-
?
adenosine + H2O
-
Streptomyces sp. inosine + NH3
-
?
adenosine + H2O
-
Streptomyces sp. J-845S inosine + NH3
-
?
adenosine-3'-monophosphate + H2O
-
Streptomyces sp. IMP-3'-monophosphate + NH3
-
?
adenosine-3'-monophosphate + H2O
-
Streptomyces sp. J-845S IMP-3'-monophosphate + NH3
-
?
cAMP + H2O
-
Streptomyces sp. ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Streptomyces sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
30°C and below Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 6
-
Streptomyces sp.

pH Range

pH Minimum pH Maximum Comment Organism
3.5 5.5
-
Streptomyces sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5 5.5 in sodium acetate buffer Streptomyces sp.