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Literature summary for 3.5.4.4 extracted from

  • Gabellieri, E.; Bernini, S.; Piras, L.; Cioni, P.; Balestreri, E.; Cercignani, G.; Felicioli, R.
    Purification, stability and kinetic properties of highly purified adenosine deaminase from Bacillus cereus NCIB 8122 (1986), Biochim. Biophys. Acta, 884, 490-496.
    View publication on PubMed
Search for more literature for 3.5.4.4

Activating Compound

Activating Compound Comment Organism Structure
NH4Cl
-
 Bacillus cereus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 adenosine
-
 Bacillus cereus
0.4
-
 2'-deoxyadenosine
-
 Bacillus cereus

Metals/Ions

Metals/Ions Comment Organism Structure
K+
-
 Bacillus cereus
Na+
-
 Bacillus cereus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53700
-
 SDS-PAGE Bacillus cereus
68000
-
 gel filtration Bacillus cereus

Organic Solvent Stability

Organic Solvent Comment Organism
dithiothreitol
-
 Bacillus cereus

Organism

Organism UniProt Comment Textmining
Bacillus cereus
-
 NCIB 8122
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus cereus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
102
-
-
 Bacillus cereus

Storage Stability

Storage Stability Organism
-20°C, KCl-buffer A, glycerol, 8 months Bacillus cereus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-deoxyadenosine + H2O
-
 Bacillus cereus 2'-deoxyinosine + NH3
-
 ?
adenosine + H2O
-
 Bacillus cereus inosine + NH3
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 10
-
 Bacillus cereus

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 11
-
 Bacillus cereus