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Literature summary for 3.5.4.34 extracted from

  • Gerber, A.; Grosjean, H.; Melcher, T.; Keller, W.
    Tad1p, a yeast tRNA-specific adenosine deaminase, is related to the mammalian pre-mRNA editing enzymes ADAR1 and ADAR2 (1998), EMBO J., 17, 4780-4789.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.4.34

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
additional information mutant strains in which the TAD1 gene is disrupted are viable but lack Tad1p enzyme activity and their tRNAAla is not modified at position A37 Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
KCl high salt concentrations (above 100 mM KCl) inhibit the reaction Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000006
-
 adenine37 in tRNAAla 30°C, pH 7,9 Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
KCl the KCl optimum for A37–I37 conversion by Tad1p is 25 mM Saccharomyces cerevisiae
Mg2+ tRNA editing is strictly magnesium dependent with an optimal concentration between 2–3 mM Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
adenine37 in tRNAAla + H2O Saccharomyces cerevisiae
-
 hypoxanthine37 in tRNAAla + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P53065
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenine37 in tRNAAla + H2O
-
 Saccharomyces cerevisiae hypoxanthine37 in tRNAAla + NH3
-
 ?
adenine37 in tRNAAla + H2O pre-mRNA and extended dsRNA are not used as substrates by Tad1p in vitro. Tad1p requires both the local conformation of the anticodon loop and a correct folding of the L-shaped tRNAAla substrate to recognize and deaminate A37 Saccharomyces cerevisiae hypoxanthine37 in tRNAAla + NH3
-
 ?

Synonyms

Synonyms Comment Organism
Tad1p/scADAT1
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0705
-
 adenine37 in tRNAAla 30°C, pH 7,9 Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
 Saccharomyces cerevisiae

General Information

General Information Comment Organism
malfunction the TAD1 gene is not essential in yeast under optimal growth conditions at 30°C Saccharomyces cerevisiae