BRENDA - Enzyme Database show
show all sequences of 3.5.4.33

Mechanism, specificity and general properties of the yeast enzyme catalysing the formation of inosine 34 in the anticodon of transfer RNA

Auxilien, S.; Crain, P.F.; Trewyn, R.W.; Grosjean, H.; J. Mol. Biol. 262, 437-458 (1996)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
5 mM abolishes activity up to 70%
Saccharomyces cerevisiae
4,7-phenanthroline
5 mM abolishes activity up to 70%
Saccharomyces cerevisiae
additional information
no inhibition by 2'-(8R)-deoxycoformycin up to 12 mM
Saccharomyces cerevisiae
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0000023
-
adenine34 in tRNASer(AGA)
pH and temperature not specified in the publication
Saccharomyces cerevisiae
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
optimum concentration2-5 mM, the enzyme does not require any cofactor, except magnesium ions, to deaminate adenosine 34 efficiently in tRNA. The dependence of the enzymatic reaction on magnesium ions probably reflects the need for a correct tRNA architecture
Saccharomyces cerevisiae
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
75000
-
gel filtration
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
adenine34 in tRNA + H2O
Saccharomyces cerevisiae
enzyme accounts for the presence of inosine 34 in all seven cytoplasmic A34-containing precursor tRNAs in yeast
hypoxanthine34 in tRNA + NH3
-
-
?
adenine34 in tRNA + H2O
Saccharomyces cerevisiae ATCC 204508
enzyme accounts for the presence of inosine 34 in all seven cytoplasmic A34-containing precursor tRNAs in yeast
hypoxanthine34 in tRNA + NH3
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Saccharomyces cerevisiae
P47058 and Q9URQ3
P47058: TAD2 and Q9URQ3: TAD3
-
Saccharomyces cerevisiae ATCC 204508
P47058 and Q9URQ3
P47058: TAD2 and Q9URQ3: TAD3
-
Purification (Commentary)
Commentary
Organism
partial
Saccharomyces cerevisiae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
adenine34 in tRNA + H2O
enzyme accounts for the presence of inosine 34 in all seven cytoplasmic A34-containing precursor tRNAs in yeast
720220
Saccharomyces cerevisiae
hypoxanthine34 in tRNA + NH3
-
-
-
?
adenine34 in tRNA + H2O
the efficacy of adenosine 34 to inosine 34 conversion depends on the nucleotide sequence of the anticodon loop and its proximal stem, the best tRNA substrates being those with a purine at position 35. The enzyme accounts for the presence of inosine 34 in all seven cytoplasmic A34-containing precursor tRNAs in yeast
720220
Saccharomyces cerevisiae
hypoxanthine34 in tRNA + NH3
-
-
-
?
adenine34 in tRNA + H2O
enzyme accounts for the presence of inosine 34 in all seven cytoplasmic A34-containing precursor tRNAs in yeast
720220
Saccharomyces cerevisiae ATCC 204508
hypoxanthine34 in tRNA + NH3
-
-
-
?
adenine34 in tRNA + H2O
the efficacy of adenosine 34 to inosine 34 conversion depends on the nucleotide sequence of the anticodon loop and its proximal stem, the best tRNA substrates being those with a purine at position 35. The enzyme accounts for the presence of inosine 34 in all seven cytoplasmic A34-containing precursor tRNAs in yeast
720220
Saccharomyces cerevisiae ATCC 204508
hypoxanthine34 in tRNA + NH3
-
-
-
?
adenine34 in tRNAAla(AGC) + H2O
-
720220
Saccharomyces cerevisiae
hypoxanthine34 in tRNAAla(IGC) + NH3
-
-
-
?
adenine34 in tRNAAla(AGC) + H2O
-
720220
Saccharomyces cerevisiae ATCC 204508
hypoxanthine34 in tRNAAla(IGC) + NH3
-
-
-
?
adenine34 in tRNASer(AGA) + H2O
-
720220
Saccharomyces cerevisiae
hypoxanthine34 in tRNASer(IGA) + NH3
-
-
-
?
adenine34 in tRNASer(AGA) + H2O
-
720220
Saccharomyces cerevisiae ATCC 204508
hypoxanthine34 in tRNASer(IGA) + NH3
-
-
-
?
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
5 mM abolishes activity up to 70%
Saccharomyces cerevisiae
4,7-phenanthroline
5 mM abolishes activity up to 70%
Saccharomyces cerevisiae
additional information
no inhibition by 2'-(8R)-deoxycoformycin up to 12 mM
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0000023
-
adenine34 in tRNASer(AGA)
pH and temperature not specified in the publication
Saccharomyces cerevisiae
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
optimum concentration2-5 mM, the enzyme does not require any cofactor, except magnesium ions, to deaminate adenosine 34 efficiently in tRNA. The dependence of the enzymatic reaction on magnesium ions probably reflects the need for a correct tRNA architecture
Saccharomyces cerevisiae
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
75000
-
gel filtration
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
adenine34 in tRNA + H2O
Saccharomyces cerevisiae
enzyme accounts for the presence of inosine 34 in all seven cytoplasmic A34-containing precursor tRNAs in yeast
hypoxanthine34 in tRNA + NH3
-
-
?
adenine34 in tRNA + H2O
Saccharomyces cerevisiae ATCC 204508
enzyme accounts for the presence of inosine 34 in all seven cytoplasmic A34-containing precursor tRNAs in yeast
hypoxanthine34 in tRNA + NH3
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
adenine34 in tRNA + H2O
enzyme accounts for the presence of inosine 34 in all seven cytoplasmic A34-containing precursor tRNAs in yeast
720220
Saccharomyces cerevisiae
hypoxanthine34 in tRNA + NH3
-
-
-
?
adenine34 in tRNA + H2O
the efficacy of adenosine 34 to inosine 34 conversion depends on the nucleotide sequence of the anticodon loop and its proximal stem, the best tRNA substrates being those with a purine at position 35. The enzyme accounts for the presence of inosine 34 in all seven cytoplasmic A34-containing precursor tRNAs in yeast
720220
Saccharomyces cerevisiae
hypoxanthine34 in tRNA + NH3
-
-
-
?
adenine34 in tRNA + H2O
enzyme accounts for the presence of inosine 34 in all seven cytoplasmic A34-containing precursor tRNAs in yeast
720220
Saccharomyces cerevisiae ATCC 204508
hypoxanthine34 in tRNA + NH3
-
-
-
?
adenine34 in tRNA + H2O
the efficacy of adenosine 34 to inosine 34 conversion depends on the nucleotide sequence of the anticodon loop and its proximal stem, the best tRNA substrates being those with a purine at position 35. The enzyme accounts for the presence of inosine 34 in all seven cytoplasmic A34-containing precursor tRNAs in yeast
720220
Saccharomyces cerevisiae ATCC 204508
hypoxanthine34 in tRNA + NH3
-
-
-
?
adenine34 in tRNAAla(AGC) + H2O
-
720220
Saccharomyces cerevisiae
hypoxanthine34 in tRNAAla(IGC) + NH3
-
-
-
?
adenine34 in tRNAAla(AGC) + H2O
-
720220
Saccharomyces cerevisiae ATCC 204508
hypoxanthine34 in tRNAAla(IGC) + NH3
-
-
-
?
adenine34 in tRNASer(AGA) + H2O
-
720220
Saccharomyces cerevisiae
hypoxanthine34 in tRNASer(IGA) + NH3
-
-
-
?
adenine34 in tRNASer(AGA) + H2O
-
720220
Saccharomyces cerevisiae ATCC 204508
hypoxanthine34 in tRNASer(IGA) + NH3
-
-
-
?
Other publictions for EC 3.5.4.33
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
718952
Su
A-to-I and C-to-U editing with ...
Agrobacterium tumefaciens, Aquifex aeolicus, Escherichia coli, Streptococcus pyogenes
Biochemistry
76
932-937
2011
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4
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9
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4
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4
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719934
Spears
A single zinc ion is sufficien ...
Trypanosoma brucei
J. Biol. Chem.
286
20366-20374
2011
-
-
-
-
11
-
-
4
-
1
-
1
-
4
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-
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1
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1
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3
1
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11
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4
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1
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1
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1
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1
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3
1
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-
4
4
721030
Ragone
The C-terminal end of the Tryp ...
Trypanosoma brucei
RNA
17
1296-1306
2011
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1
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9
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6
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1
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1
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1
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6
1
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1
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9
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6
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-
1
-
1
-
-
6
1
-
-
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-
-
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-
6
6
713269
Delannoy
Arabidopsis tRNA adenosine dea ...
Arabidopsis thaliana
Plant Cell
21
2058-2071
2009
-
-
1
-
-
-
-
-
2
-
-
1
-
7
-
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2
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1
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1
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2
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1
1
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-
721023
Karcher
Identification of the chloropl ...
Arabidopsis thaliana
RNA
15
1251-1257
2009
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1
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1
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1
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6
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1
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1
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1
1
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698470
Luo
Transition state structure of ...
Escherichia coli
J. Am. Chem. Soc.
130
2649-2655
2008
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1
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1
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719843
Tsutsumi
Wobble inosine tRNA modificati ...
Schizosaccharomyces pombe
J. Biol. Chem.
282
33459-33465
2007
-
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4
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1
1
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720892
Rubio
An adenosine-to-inosine tRNA-e ...
Trypanosoma brucei
Proc. Natl. Acad. Sci. USA
104
7821-7826
2007
-
-
-
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1
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4
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721010
Lee
Crystal structure of the tRNA- ...
Streptococcus pyogenes
Proteins
68
1016-1019
2007
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1
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1
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689140
Losey
Crystal structure of Staphyloc ...
Staphylococcus aureus
Nat. Struct. Mol. Biol.
13
153-159
2006
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1
1
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1
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1
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669323
Kuratani
Crystal structure of tRNA aden ...
Aquifex aeolicus
J. Biol. Chem.
280
16002-16008
2005
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1
1
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1
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1
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1
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718485
Ku
Crystallization and preliminar ...
Streptococcus pyogenes, Streptococcus pyogenes ATCC BAA-946
Acta Crystallogr. Sect. F
61
375-357
2005
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1
1
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5
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718839
Elias
Biochemical and structural stu ...
Agrobacterium tumefaciens, Aquifex aeolicus, Escherichia coli, Saccharomyces cerevisiae, Saccharomyces cerevisiae ATCC 204508
Biochemistry
44
12057-12065
2005
-
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4
1
4
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1
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5
-
14
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4
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10
4
4
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4
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4
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1
4
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1
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5
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4
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10
4
4
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4
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719356
Wolf
tadA, an essential tRNA-specif ...
Escherichia coli
EMBO J.
21
3841-3851
2002
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1
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721050
Gerber
An adenosine deaminase that ge ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae ATCC 204508
Science
286
1146-1149
1999
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720220
Auxilien
Mechanism, specificity and gen ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae ATCC 204508
J. Mol. Biol.
262
437-458
1996
-
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3
1
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1
1
2
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7
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1
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8
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3
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1
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1
2
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1
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8
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