BRENDA - Enzyme Database show
show all sequences of 3.5.4.33

tadA, an essential tRNA-specific adenosine deaminase from Escherichia coli

Wolf, J.; Gerber, A.P.; Keller, W.; EMBO J. 21, 3841-3851 (2002)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Escherichia coli
Engineering
Amino acid exchange
Commentary
Organism
D64E
D64E abolishes the enzymatic activity of tadA in vitro, it does not detectably affect its activity in cells
Escherichia coli
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
18718
-
2 * 18718, can form a homodimer in vitro, it is not proved that it is active as a homodimer in vitro, calculated from sequence
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
adenine34 in tRNAArg + H2O
Escherichia coli
the enzyme catalyzes the site-specific inosine formation at the wobble position (position 34) of tRNAArg2, the only tRNA having this modification in prokaryotes. tadA is an essential gene in Escherichiy coli
hypoxanthine34 in tRNAArg + NH3
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
P68398
-
-
Purification (Commentary)
Commentary
Organism
-
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
adenine34 in tRNAArg + H2O
the enzyme catalyzes the site-specific inosine formation at the wobble position (position 34) of tRNAArg2, the only tRNA having this modification in prokaryotes. tadA is an essential gene in Escherichiy coli
719356
Escherichia coli
hypoxanthine34 in tRNAArg + NH3
-
-
-
?
adenine34 in tRNAArg + H2O
the enzyme catalyzes the site-specific inosine formation at the wobble position (position 34) of tRNAArg2, the only tRNA having this modification in prokaryotes. With the exception of yeast tRNAArg, no other eukaryotic tRNA substrates are found to be modified by tadA. An artificial yeast tRNAAsp, which carries the anticodon loop of yeast tRNAArg, is bound and modified by tadA. A tRNAArg2 minisubstrate containing the anticodon stem and loop is sufficient for specific deamination by tadA. Nucleotides at positions 33-36 are sufficient for inosine formation in mutant Arg2 minisubstrates. The anticodon is thus a major determinant for tadA substrate specificity
719356
Escherichia coli
hypoxanthine34 in tRNAArg + NH3
-
-
-
?
Subunits
Subunits
Commentary
Organism
homodimer
2 * 18718, can form a homodimer in vitro, it is not proved that it is active as a homodimer in vitro, calculated from sequence
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Escherichia coli
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
D64E
D64E abolishes the enzymatic activity of tadA in vitro, it does not detectably affect its activity in cells
Escherichia coli
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
18718
-
2 * 18718, can form a homodimer in vitro, it is not proved that it is active as a homodimer in vitro, calculated from sequence
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
adenine34 in tRNAArg + H2O
Escherichia coli
the enzyme catalyzes the site-specific inosine formation at the wobble position (position 34) of tRNAArg2, the only tRNA having this modification in prokaryotes. tadA is an essential gene in Escherichiy coli
hypoxanthine34 in tRNAArg + NH3
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
adenine34 in tRNAArg + H2O
the enzyme catalyzes the site-specific inosine formation at the wobble position (position 34) of tRNAArg2, the only tRNA having this modification in prokaryotes. tadA is an essential gene in Escherichiy coli
719356
Escherichia coli
hypoxanthine34 in tRNAArg + NH3
-
-
-
?
adenine34 in tRNAArg + H2O
the enzyme catalyzes the site-specific inosine formation at the wobble position (position 34) of tRNAArg2, the only tRNA having this modification in prokaryotes. With the exception of yeast tRNAArg, no other eukaryotic tRNA substrates are found to be modified by tadA. An artificial yeast tRNAAsp, which carries the anticodon loop of yeast tRNAArg, is bound and modified by tadA. A tRNAArg2 minisubstrate containing the anticodon stem and loop is sufficient for specific deamination by tadA. Nucleotides at positions 33-36 are sufficient for inosine formation in mutant Arg2 minisubstrates. The anticodon is thus a major determinant for tadA substrate specificity
719356
Escherichia coli
hypoxanthine34 in tRNAArg + NH3
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 18718, can form a homodimer in vitro, it is not proved that it is active as a homodimer in vitro, calculated from sequence
Escherichia coli
General Information
General Information
Commentary
Organism
malfunction
disruption of the wild-type chromosomal copy of tadA results in viable Escherichia coli only when an additional plasmid-borne tadA gene is also present. TadA is essential for viability of eubacterial organisms
Escherichia coli
General Information (protein specific)
General Information
Commentary
Organism
malfunction
disruption of the wild-type chromosomal copy of tadA results in viable Escherichia coli only when an additional plasmid-borne tadA gene is also present. TadA is essential for viability of eubacterial organisms
Escherichia coli
Other publictions for EC 3.5.4.33
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
718952
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719934
Spears
A single zinc ion is sufficien ...
Trypanosoma brucei
J. Biol. Chem.
286
20366-20374
2011
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11
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4
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721030
Ragone
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9
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6
1
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6
6
713269
Delannoy
Arabidopsis tRNA adenosine dea ...
Arabidopsis thaliana
Plant Cell
21
2058-2071
2009
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1
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2
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7
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721023
Karcher
Identification of the chloropl ...
Arabidopsis thaliana
RNA
15
1251-1257
2009
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698470
Luo
Transition state structure of ...
Escherichia coli
J. Am. Chem. Soc.
130
2649-2655
2008
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719843
Tsutsumi
Wobble inosine tRNA modificati ...
Schizosaccharomyces pombe
J. Biol. Chem.
282
33459-33465
2007
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720892
Rubio
An adenosine-to-inosine tRNA-e ...
Trypanosoma brucei
Proc. Natl. Acad. Sci. USA
104
7821-7826
2007
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721010
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Crystal structure of the tRNA- ...
Streptococcus pyogenes
Proteins
68
1016-1019
2007
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689140
Losey
Crystal structure of Staphyloc ...
Staphylococcus aureus
Nat. Struct. Mol. Biol.
13
153-159
2006
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1
1
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1
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669323
Kuratani
Crystal structure of tRNA aden ...
Aquifex aeolicus
J. Biol. Chem.
280
16002-16008
2005
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1
1
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718485
Ku
Crystallization and preliminar ...
Streptococcus pyogenes, Streptococcus pyogenes ATCC BAA-946
Acta Crystallogr. Sect. F
61
375-357
2005
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718839
Elias
Biochemical and structural stu ...
Agrobacterium tumefaciens, Aquifex aeolicus, Escherichia coli, Saccharomyces cerevisiae, Saccharomyces cerevisiae ATCC 204508
Biochemistry
44
12057-12065
2005
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1
4
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1
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5
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14
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10
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10
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719356
Wolf
tadA, an essential tRNA-specif ...
Escherichia coli
EMBO J.
21
3841-3851
2002
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1
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721050
Gerber
An adenosine deaminase that ge ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae ATCC 204508
Science
286
1146-1149
1999
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720220
Auxilien
Mechanism, specificity and gen ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae ATCC 204508
J. Mol. Biol.
262
437-458
1996
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