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Literature summary for 3.5.4.33 extracted from

  • Wolf, J.; Gerber, A.P.; Keller, W.
    tadA, an essential tRNA-specific adenosine deaminase from Escherichia coli (2002), EMBO J., 21, 3841-3851.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.4.33

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Escherichia coli

Protein Variants

Protein Variants Comment Organism
D64E D64E abolishes the enzymatic activity of tadA in vitro, it does not detectably affect its activity in cells Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18718
-
 2 * 18718, can form a homodimer in vitro, it is not proved that it is active as a homodimer in vitro, calculated from sequence Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
adenine34 in tRNAArg + H2O Escherichia coli the enzyme catalyzes the site-specific inosine formation at the wobble position (position 34) of tRNAArg2, the only tRNA having this modification in prokaryotes. tadA is an essential gene in Escherichiy coli hypoxanthine34 in tRNAArg + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P68398
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenine34 in tRNAArg + H2O the enzyme catalyzes the site-specific inosine formation at the wobble position (position 34) of tRNAArg2, the only tRNA having this modification in prokaryotes. tadA is an essential gene in Escherichiy coli Escherichia coli hypoxanthine34 in tRNAArg + NH3
-
 ?
adenine34 in tRNAArg + H2O the enzyme catalyzes the site-specific inosine formation at the wobble position (position 34) of tRNAArg2, the only tRNA having this modification in prokaryotes. With the exception of yeast tRNAArg, no other eukaryotic tRNA substrates are found to be modified by tadA. An artificial yeast tRNAAsp, which carries the anticodon loop of yeast tRNAArg, is bound and modified by tadA. A tRNAArg2 minisubstrate containing the anticodon stem and loop is sufficient for specific deamination by tadA. Nucleotides at positions 33-36 are sufficient for inosine formation in mutant Arg2 minisubstrates. The anticodon is thus a major determinant for tadA substrate specificity Escherichia coli hypoxanthine34 in tRNAArg + NH3
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 ?

Subunits

Subunits Comment Organism
homodimer 2 * 18718, can form a homodimer in vitro, it is not proved that it is active as a homodimer in vitro, calculated from sequence Escherichia coli

Synonyms

Synonyms Comment Organism
TadA
-
 Escherichia coli
tadA/ecADAT2
-
 Escherichia coli

General Information

General Information Comment Organism
malfunction disruption of the wild-type chromosomal copy of tadA results in viable Escherichia coli only when an additional plasmid-borne tadA gene is also present. TadA is essential for viability of eubacterial organisms Escherichia coli