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Literature summary for 3.5.4.30 extracted from

  • Oehlenschlaeger, C.B.; L?vgreen, M.N.; Reinauer, E.; Lehtinen, E.; Pind, M.L.; Harris, P.; Martinussen, J.; Willemoes, M.
    Bacillus halodurans strain C125 encodes and synthesizes enzymes from both known pathways to form dump directly from cytosine deoxyribonucleotides (2015), Appl. Environ. Microbiol., 81, 3395-3404.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.4.30

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Halalkalibacterium halodurans

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with dTTP, hanging drop vapor diffusion method, using 200 mM lithium citrate and 20% (w/v) polyethylene glycol 4000 Halalkalibacterium halodurans

Inhibitors

Inhibitors Comment Organism Structure
dTTP
-
 Halalkalibacterium halodurans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dCTP + H2O Halalkalibacterium halodurans
-
 dUMP + diphosphate + NH3
-
 ?
dCTP + H2O Halalkalibacterium halodurans C-125
-
 dUMP + diphosphate + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Halalkalibacterium halodurans Q9KFV3
-
-
Halalkalibacterium halodurans C-125 Q9KFV3
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and DE-52 anion-exchange column chromatography Halalkalibacterium halodurans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dCTP + H2O
-
 Halalkalibacterium halodurans dUMP + diphosphate + NH3
-
 ?
dCTP + H2O
-
 Halalkalibacterium halodurans C-125 dUMP + diphosphate + NH3
-
 ?

Subunits

Subunits Comment Organism
homotrimer x-ray crystallography Halalkalibacterium halodurans

Synonyms

Synonyms Comment Organism
DCD:DUT bifunctional enzyme with dCTP deaminase and dUTPase activities Halalkalibacterium halodurans
dcdB
-
 Halalkalibacterium halodurans