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Literature summary for 3.5.4.3 extracted from

  • Fernandez, J.R.; Welsh, W.J.; Firestein, B.L.
    Structural characterization of the zinc binding domain in cytosolic PSD-95 interactor (cypin): Role of zinc binding in guanine deamination and dendrite branching (2008), Proteins, 70, 873-881.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.4.3

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in COS-7 cells, subcloning in Escherichia coli strain DH5alpha Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
D330A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Rattus norvegicus
H240A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Rattus norvegicus
H279A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Rattus norvegicus
H71A site-directed mutagenesis, the mutant shows slightly reduced activity compared to the wild-type enzyme Rattus norvegicus
H82A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Rattus norvegicus
H84A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Rattus norvegicus
I83A site-directed mutagenesis, the mutant shows slightly reduced activity compared to the wild-type enzyme Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Rattus norvegicus 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ essential for activity, binding structure, computational homology modeling shows that several histidines and aspartic acid residues are responsible for zinc binding, e.g. His82, His84, His240, His279, and Asp330, overview Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
guanine + H2O Rattus norvegicus the enzyme plays a central role in neuronal development, and an active role in regulating dendrite branching in hippocampal neurons, cypin-promoted increases in dendrite number are dependent on its zinc-dependent guanine deaminase activity, overview xanthine + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Rattus norvegicus
-
hippocampus
-
 Rattus norvegicus
-
neuron
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
guanine + H2O
-
 Rattus norvegicus xanthine + NH3
-
 ?
guanine + H2O the enzyme plays a central role in neuronal development, and an active role in regulating dendrite branching in hippocampal neurons, cypin-promoted increases in dendrite number are dependent on its zinc-dependent guanine deaminase activity, overview Rattus norvegicus xanthine + NH3
-
 ?

Subunits

Subunits Comment Organism
More computational homology modeling techniques to construct a three dimensional structural model of cypin, overview Rattus norvegicus

Synonyms

Synonyms Comment Organism
cypin
-
 Rattus norvegicus
cytosolic PSD-95 interactor
-
 Rattus norvegicus
More cypin is a member of the metal-dependent aminohydrolase family Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Rattus norvegicus