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Literature summary for 3.5.4.3 extracted from

  • Maynes, J.T.; Yuan, R.G.; Snyder, F.F.
    Identification, expression and characterization of Escherichia coli guanine deaminase (2000), J. Bacteriol., 8, 4658-4660.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.4.3

Cloned(Commentary)

Cloned (Comment) Organism
-
 Homo sapiens
gene identified with human cDNA, amplified, subcloned into pMAL-c2 vector, expressed and purified Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
 guanine recombinant protein Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ association of 1 atom per enzyme monomer Escherichia coli
Zn2+ association of 1 atom per enzyme monomer Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50200
-
 recombinant protein Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
guanine + H2O Escherichia coli
-
 xanthine + NH3
-
 ?
guanine + H2O Homo sapiens
-
 xanthine + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Homo sapiens Q9Y2T3 human
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
guanine + H2O
-
 Escherichia coli xanthine + NH3
-
 ?
guanine + H2O
-
 Homo sapiens xanthine + NH3
-
 ?

Synonyms

Synonyms Comment Organism
E. coli guanine deaminase
-
 Escherichia coli