Any feedback?
Literature summary for 3.5.4.3 extracted from
- Rabbit liver guanine deaminase. Chemical, physical, and kinetic properties (1974), J. Biol. Chem., 249, 3862-3866.
General Stability
| General Stability | Organism |
|---|---|
| stabilized by 2-mercaptoethanol, labile to heat | Oryctolagus cuniculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5-aminoimidazole-4-carboxamide | - |
Oryctolagus cuniculus |  |
| 5-aminoimidazole-4-carboxamide | - |
Rattus norvegicus | |
| 5-aminoimidazole-4-carboxamide ribonucleoside | - |
Oryctolagus cuniculus | |
| 5-aminoimidazole-4-carboxamide ribonucleoside | - |
Rattus norvegicus | |
| iodoacetamide | - |
Oryctolagus cuniculus | |
| iodoacetic acid | - |
Oryctolagus cuniculus | |
| p-hydroxymercuribenzoate | - |
Oryctolagus cuniculus | |
| p-hydroxymercuribenzoate | - |
Rattus norvegicus | |
| tetrahydrofolic acid | - |
Oryctolagus cuniculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0125 | - |
guanine | - |
Oryctolagus cuniculus | |
| 0.0182 | - |
guanine | buffer with 50% D2O | Oryctolagus cuniculus | |
| 0.025 | - |
guanine | buffer with 100% D2O | Oryctolagus cuniculus | |
| 0.333 | - |
8-Azaguanine | at pH 6 | Oryctolagus cuniculus | |
| 0.8 | - |
6-thioguanine | - |
Oryctolagus cuniculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54000 | - |
SDS-PAGE | Oryctolagus cuniculus |
| 55000 | - |
liver, single polypeptide chain, gel filtration | Rattus norvegicus |
| 55000 | - |
liver, single polypeptide chain, gel filtration | Oryctolagus cuniculus |
| 56000 | - |
liver enzyme, gel filtration | Oryctolagus cuniculus |
| 170000 | - |
- |
Oryctolagus cuniculus |
| 525000 | - |
- |
Oryctolagus cuniculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| guanine + H2O | Rattus norvegicus | - |
xanthine + NH3 | - |
? | |
| guanine + H2O | Oryctolagus cuniculus | - |
xanthine + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryctolagus cuniculus | - |
rabbit | - |
| Rattus norvegicus | - |
rat | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Oryctolagus cuniculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
| liver | - |
Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 25.2 | - |
liver enzyme | Oryctolagus cuniculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-thioguanine + H2O | - |
Oryctolagus cuniculus | 6-thioxanthine + NH3 | - |
? | |
| 8-azaguanine + H2O | - |
Oryctolagus cuniculus | 8-azaxanthine + NH3 | - |
? | |
| guanine + H2O | - |
Rattus norvegicus | xanthine + NH3 | - |
? | |
| guanine + H2O | - |
Oryctolagus cuniculus | xanthine + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Rattus norvegicus |
| monomer | 1 * 55000, gel filtration, SDS-PAGE | Oryctolagus cuniculus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 50 | activation energy linear | Oryctolagus cuniculus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Rattus norvegicus |
| 50 | - |
relatively stable up to 50°C | Oryctolagus cuniculus |
| 55 | - |
42% loss of enzyme activity substained upon incubation for 25 min | Oryctolagus cuniculus |
| 55 | - |
loss of catalytic activity upon incubation for 20 min | Oryctolagus cuniculus |
| 60 | - |
- |
Rattus norvegicus |
| 60 | - |
irreversible denaturation upon incubation for 25 min | Oryctolagus cuniculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
8-azaguanine as substrate | Oryctolagus cuniculus |
| 6.8 | - |
pH optimum is independent of the buffer system | Oryctolagus cuniculus |
| 7.7 | - |
- |
Oryctolagus cuniculus |
| 8 | - |
- |
Rattus norvegicus |
| 8 | - |
- |
Oryctolagus cuniculus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
