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Literature summary for 3.5.4.27 extracted from
- Activation and thermostabilization effects of cyclic 2,3-diphosphoglycerate on enzymes from the hyperthermophilic Methanopyrus kandleri (1998), Arch. Microbiol., 170, 469-472 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2,3-bisphosphoglycerate | optimally active at 0.6 M, about 5500 U/mg | Methanopyrus kandleri |  |
| cyclic 2,3-bisphosphoglycerate | optimally active at 0.75 M, about 7500 U/mg | Methanopyrus kandleri | |
| phosphate | optimally active at 1.5 M, about 9000 U/mg | Methanopyrus kandleri | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O | Methanopyrus kandleri | - |
5-formyl-5,6,7,8-tetrahydromethanopterin | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanopyrus kandleri | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | growth temperature optimum is 98°C | Methanopyrus kandleri | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O | - |
Methanopyrus kandleri | 5-formyl-5,6,7,8-tetrahydromethanopterin | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cyclohydrolase | - |
Methanopyrus kandleri |
| N5,N10-methenyltetrahydromethanopterin cyclohydrolase | - |
Methanopyrus kandleri |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
assay at | Methanopyrus kandleri |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
enzyme in 40 mM potassium phosphate, pH 8.0, 1 h, 8% activity remaining, with addition of 0.5 M cyclic 2,3-bisphosphoglycerate or 0.7 M bisphosphoglycerate or 1.5 M phosphate, 100% activity are remaining | Methanopyrus kandleri |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Methanopyrus kandleri |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | in hyperthermophilic methanogens of the orders Methanobacteriales and Methanopyrales, the cyclic 2,3-bisphosphoglycerate (cDPG) concentration appears to correlate with the growth temperature optimum, the concentration being 1 M in Methanopyrus kandleri (98°C) | Methanopyrus kandleri |
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