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Literature summary for 3.5.4.27 extracted from
- A reversed genetic approach reveals the coenzyme specificity and other catalytic properties of three enzymes putatively involved in anaerobic oxidation of methane with sulfate (2014), Environ. Microbiol., 16, 3431-3442.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | uncultured archaeon |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.06 | - |
5,10-methenyl-5,6,7,8-tetrahydromethanopterin | pH 7.6, 10°C | uncultured archaeon |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | activity in cell extract is dependent on the presence of high concentrations of potassium phosphate. The activity in 1.0 M potassium phosphate buffer pH 7.6 is eightfold higher than that in 0.12 M potassium phosphate buffer pH 7.6 | uncultured archaeon | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34982 | - |
x * 34982, calculated from sequence | uncultured archaeon |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O | uncultured archaeon | - |
5-formyl-5,6,7,8-tetrahydromethanopterin | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| uncultured archaeon | D1JGU3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
uncultured archaeon |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O | - |
uncultured archaeon | 5-formyl-5,6,7,8-tetrahydromethanopterin | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 34982, calculated from sequence | uncultured archaeon |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CBH37751 | - |
uncultured archaeon |
| Mch | - |
uncultured archaeon |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
uncultured archaeon |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 70 | 30°C: about 75% of maximal activity, 70°C: about 55% of maximal activity | uncultured archaeon |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
assay at | uncultured archaeon |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| uncultured archaeon | calculated from sequence | - |
4.3 |
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Release 2023.1 (January 2023)
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