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Literature summary for 3.5.4.27 extracted from

  • Shima, S.; Herault, D.A.; Berkessel, A.; Thauer, R.K.
    Activation and thermostabilization effects of cyclic 2,3-diphosphoglycerate on enzymes from the hyperthermophilic Methanopyrus kandleri (1998), Arch. Microbiol., 170, 469-472.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2,3-bisphosphoglycerate the potassium salts of 2,3-bisphosphoglycerate stimulates cyclohydrolase activity Methanopyrus kandleri
2,3-diphosphoglycerate the activity increases with increasing 2,3-diphosphoglycerate concentrations from 30 U/mg up to a specific activity of 7,500 U/mg. The maximal activity is reached at a 2,3-diphosphoglycerate concentration of 0.75 M. At higher concentrations, the activity decreases Methanopyrus kandleri
phosphate the potassium salts of phosphateglycerate stimulates cyclohydrolase activity Methanopyrus kandleri

General Stability

General Stability Organism
at cyclic 2,3-diphosphoglycerate concentrations prevailing in the cells of Methanopyrus kandleri the enzyme is completely thermostable. At molar concentrations also the potassium salts of phosphate and of 2,3-bisphosphoglycerate, the biosynthetic precursor of cyclic 2,3-diphosphoglycerate confer thermostability to the enzymes Methanopyrus kandleri

Organism

Organism UniProt Comment Textmining
Methanopyrus kandleri
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-
-

Oxidation Stability

Oxidation Stability Organism
stable under oxic conditions Methanopyrus kandleri

Synonyms

Synonyms Comment Organism
N5,N10-methenyltetrahydromethanopterin cyclohydrolase
-
Methanopyrus kandleri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
assay at Methanopyrus kandleri

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
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at cyclic 2,3-diphosphoglycerate concentrations prevailing in the cells of Methanopyrus kandleri the enzyme is completely thermostable. At molar concentrations also the potassium salts of phosphate and of 2,3-bisphosphoglycerate, the biosynthetic precursor of cyclic 2,3-diphosphoglycerate confer thermostability to the enzymes Methanopyrus kandleri
90
-
in 0.75 M 2,3-diphosphoglycerate, the cyclohydrolase is completely stable at pH 8.0 and 90°C Methanopyrus kandleri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Methanopyrus kandleri

pH Stability

pH Stability pH Stability Maximum Comment Organism
8
-
in 0.75 M 2,3-diphosphoglycerate, the cyclohydrolase is completely stable at pH 8.0 and 90°C Methanopyrus kandleri