Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2,3-bisphosphoglycerate | the potassium salts of 2,3-bisphosphoglycerate stimulates cyclohydrolase activity | Methanopyrus kandleri | |
2,3-diphosphoglycerate | the activity increases with increasing 2,3-diphosphoglycerate concentrations from 30 U/mg up to a specific activity of 7,500 U/mg. The maximal activity is reached at a 2,3-diphosphoglycerate concentration of 0.75 M. At higher concentrations, the activity decreases | Methanopyrus kandleri | |
phosphate | the potassium salts of phosphateglycerate stimulates cyclohydrolase activity | Methanopyrus kandleri |
General Stability | Organism |
---|---|
at cyclic 2,3-diphosphoglycerate concentrations prevailing in the cells of Methanopyrus kandleri the enzyme is completely thermostable. At molar concentrations also the potassium salts of phosphate and of 2,3-bisphosphoglycerate, the biosynthetic precursor of cyclic 2,3-diphosphoglycerate confer thermostability to the enzymes | Methanopyrus kandleri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanopyrus kandleri | - |
- |
- |
Oxidation Stability | Organism |
---|---|
stable under oxic conditions | Methanopyrus kandleri |
Synonyms | Comment | Organism |
---|---|---|
N5,N10-methenyltetrahydromethanopterin cyclohydrolase | - |
Methanopyrus kandleri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
assay at | Methanopyrus kandleri |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
at cyclic 2,3-diphosphoglycerate concentrations prevailing in the cells of Methanopyrus kandleri the enzyme is completely thermostable. At molar concentrations also the potassium salts of phosphate and of 2,3-bisphosphoglycerate, the biosynthetic precursor of cyclic 2,3-diphosphoglycerate confer thermostability to the enzymes | Methanopyrus kandleri |
90 | - |
in 0.75 M 2,3-diphosphoglycerate, the cyclohydrolase is completely stable at pH 8.0 and 90°C | Methanopyrus kandleri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Methanopyrus kandleri |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | - |
in 0.75 M 2,3-diphosphoglycerate, the cyclohydrolase is completely stable at pH 8.0 and 90°C | Methanopyrus kandleri |