Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Methanopyrus kandleri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
N5,N10-methenyl-5,6,7,8-tetrahydromethanopterin | recombinant enzyme | Methanopyrus kandleri | |
0.04 | - |
N5,N10-methenyl-5,6,7,8-tetrahydromethanopterin | - |
Methanopyrus kandleri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
? * 41000, SDS-PAGE | Methanopyrus kandleri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O | Methanosarcina barkeri | - |
? | - |
? | |
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O | Methanopyrus kandleri | - |
? | - |
? | |
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O | Methanothermobacter thermautotrophicus | enzyme is involved in methanogenesis from CO2 and H2, which represents the energy metabolism | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | - |
- |
- |
Methanopyrus kandleri | - |
- |
- |
Methanosarcina barkeri | - |
- |
- |
Methanothermobacter thermautotrophicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanopyrus kandleri |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5410 | - |
- |
Methanopyrus kandleri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O | - |
Methanosarcina barkeri | N5-formyl-5,6,7,8-tetrahydromethanopterin | - |
? | |
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O | - |
Methanothermobacter thermautotrophicus | N5-formyl-5,6,7,8-tetrahydromethanopterin | - |
? | |
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O | - |
Methanocaldococcus jannaschii | N5-formyl-5,6,7,8-tetrahydromethanopterin | - |
? | |
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O | - |
Methanopyrus kandleri | N5-formyl-5,6,7,8-tetrahydromethanopterin | - |
? | |
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O | - |
Methanosarcina barkeri | ? | - |
? | |
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O | - |
Methanopyrus kandleri | ? | - |
? | |
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O | enzyme is involved in methanogenesis from CO2 and H2, which represents the energy metabolism | Methanothermobacter thermautotrophicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | ? * 41000, SDS-PAGE | Methanopyrus kandleri |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
pH 8.0, 40 mM K2HPO4, stable up to | Methanopyrus kandleri |