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Literature summary for 3.5.4.27 extracted from

  • Dimarco, A.A.; Donnelly, M.I.; Wolfe, R.S.
    Purification and properties of the 5,10-methenyltetrahydromethanopterin cyclohydrolase from Methanobacterium thermoautotrophicum (1986), J. Bacteriol., 168, 1372-1377.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
10-Formyltetrahydromethanopterin
-
Methanothermobacter thermautotrophicus
Mn(CH3COO)2
-
Methanothermobacter thermautotrophicus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ stimulates Methanothermobacter thermautotrophicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
2 * 41000, SDS-PAGE Methanothermobacter thermautotrophicus
82000
-
gel filtration Methanothermobacter thermautotrophicus

Organism

Organism UniProt Comment Textmining
Methanothermobacter thermautotrophicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Methanothermobacter thermautotrophicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O the methenyl derivative is favored over 5-formyltetrahydromethanopterin Methanothermobacter thermautotrophicus N5-formyl-5,6,7,8-tetrahydromethanopterin
-
r

Subunits

Subunits Comment Organism
dimer 2 * 41000, SDS-PAGE Methanothermobacter thermautotrophicus