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Literature summary for 3.5.4.25 extracted from

  • Schramek, N.; Bracher, A.; Bacher, A.
    Biosynthesis of riboflavin. Single turnover kinetic analysis of GTP cyclohydrolase II (2001), J. Biol. Chem., 276, 44157-44162.
    View publication on PubMed
Search for more literature for 3.5.4.25

Application

Application Comment Organism
pharmacology enzyme is a potential drug target, since numerous pathogenic microorganims are absolutely dependent on endogenous synthesis of riboflavin, target for development of bactericidal inhibitors Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information pre-steady state kinetics, single turnover experiments, stopped-flow experiments Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + H2O Escherichia coli first committed step in the biosynthesis of riboflavin, overview formate + 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Reaction

Reaction Comment Organism Reaction ID
GTP + 4 H2O = formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + 2 phosphate reaction mechanism Escherichia coli
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone triphosphate + 2 H2O compound does serve as a substrate but not as kinetically competent intermediate Escherichia coli formate + 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + diphosphate
-
 ?
GTP + H2O
-
 Escherichia coli formate + 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + diphosphate GMP is also formed as a minor product ?
GTP + H2O first committed step in the biosynthesis of riboflavin, overview Escherichia coli formate + 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + diphosphate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.064
-
 GTP pH 8.5, 30°C Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 assay at Escherichia coli