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Literature summary for 3.5.4.23 extracted from

  • Kumasaka, T.; Yamamoto, M.; Furuichi, M.; Nakasako, M.; Teh, A.H.; Kimura, M.; Yamaguchi, I.; Ueki, T.
    Crystal structures of blasticidin S deaminase (BSD): implications for dynamic properties of catalytic zinc (2007), J. Biol. Chem., 282, 37103-37111.
    View publication on PubMed
Search for more literature for 3.5.4.23

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Aspergillus terreus

Crystallization (Commentary)

Crystallization (Comment) Organism
using 20% (w/v) PEG8000, 50 mM magnesium chloride, and 0.1 M sodium cacodylate at pH 7.0 as precipitant Aspergillus terreus

Protein Variants

Protein Variants Comment Organism
E56Q marginally active Aspergillus terreus
H48Q no catalytic activity Aspergillus terreus
R90K has a similar structure to the native enzyme except for the tetramer interface region Aspergillus terreus

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ dependent, contains 1 Zn2+ per subunit Aspergillus terreus

Organism

Organism UniProt Comment Textmining
Aspergillus terreus P0C2P0
-
-
Aspergillus terreus S-712 / ATCC 28865 P0C2P0
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus terreus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
blasticidin S + H2O
-
 Aspergillus terreus deaminohydroxyblasticidin S + NH3
-
 ?
blasticidin S + H2O
-
 Aspergillus terreus S-712 / ATCC 28865 deaminohydroxyblasticidin S + NH3
-
 ?

Subunits

Subunits Comment Organism
homotetramer x-ray crystallography Aspergillus terreus

Synonyms

Synonyms Comment Organism
blasticidin S deaminase
-
 Aspergillus terreus
BSD
-
 Aspergillus terreus