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Literature summary for 3.5.4.23 extracted from

  • Fukuda, H.; Kizaki, Y.
    A new transformation system of Saccharomyces cerevisiae with blasticidin S deaminase gene (1999), Biotechnol. Lett., 21, 969-971.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
bsd overexpressed in Escherichia coli BL 21 Aspergillus terreus
PCR amplification of the BSD gene, cloned from cDNA and expressed in Escherichia coli, also transformed into Schizosaccharomyces pombe and Pyricularia oryzae, P. oryzae could not be transformed with bsr, but with BSD Aspergillus terreus
plasmid pAUR123-BSD, transformation of industrial yeast Saccharomyces cerevisiae X-2180AB Aspergillus terreus
selectable marker for mammalian cells Aspergillus terreus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
blasticidin S + H2O Aspergillus terreus
-
deaminohydroxyblasticidin S + NH3
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus terreus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Aspergillus terreus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
blasticidin S + H2O
-
Aspergillus terreus deaminohydroxyblasticidin S + NH3
-
?