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Literature summary for 3.5.4.22 extracted from

  • Watanabe, S.; Morimoto, D.; Fukumori, F.; Shinomiya, H.; Nishiwaki, H.; Kawano-Kawada, M.; Sasai, Y.; Tozawa, Y.; Watanabe, Y.
    Identification and characterization of D-hydroxyproline dehydrogenase and DELTA1-pyrroline-4-hydroxy-2-carboxylate deaminase involved in novel L-hydroxyproline metabolism of bacteria: metabolic convergent evolution (2012), J. Biol. Chem., 287, 32674-32688.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.4.22

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH5alpha cells Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
L-2-oxo-3-deoxyarabinonate competitive inhibition Pseudomonas putida
pyruvate competitive inhibition Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.693
-
 1-pyrroline-4-hydroxy-2-carboxylate at pH 8.0 and 30°C Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 8 * 35000, His6-tagged protein, SDS-PAGE Pseudomonas putida
35342
-
 8 * 35342, calculated from amino acid sequence Pseudomonas putida
275000
-
 gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-pyrroline-4-hydroxy-2-carboxylate + H2O Pseudomonas putida
-
 2,5-dioxopentanoate + NH3
-
 r
1-pyrroline-4-hydroxy-2-carboxylate + H2O Pseudomonas putida KT 2442
-
 2,5-dioxopentanoate + NH3
-
 r

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida KT 2442
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-pyrroline-4-hydroxy-2-carboxylate + H2O
-
 Pseudomonas putida 2,5-dioxopentanoate + NH3
-
 r
1-pyrroline-4-hydroxy-2-carboxylate + H2O
-
 Pseudomonas putida KT 2442 2,5-dioxopentanoate + NH3
-
 r

Subunits

Subunits Comment Organism
homooctamer 8 * 35000, His6-tagged protein, SDS-PAGE Pseudomonas putida
homooctamer 8 * 35342, calculated from amino acid sequence Pseudomonas putida

Synonyms

Synonyms Comment Organism
DELTA1-pyrroline-4-hydroxy-2-carboxylate deaminase
-
 Pseudomonas putida
LhpC
-
 Pseudomonas putida
Pyr4H2C deaminase
-
 Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.19
-
 1-pyrroline-4-hydroxy-2-carboxylate at pH 8.0 and 30°C Pseudomonas putida

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.492
-
 pyruvate at pH 8.0 and 30°C Pseudomonas putida
0.592
-
 L-2-oxo-3-deoxyarabinonate at pH 8.0 and 30°C Pseudomonas putida

General Information

General Information Comment Organism
malfunction disruption of the enzyme genes leads to loss of growth on L-hydroxyproline (as well as D-hydroxyproline) but not L- and D-proline Pseudomonas putida