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Literature summary for 3.5.4.22 extracted from
- Enzymatic deamination of DELTA1-pyrroline-4-hydroxy-2carboxylate to 2,5-dioxovalerate (alpha-ketoglutaric semialdehyde) (1965), J. Biol. Chem., 240, 4344-4351.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 0.01 mM | Pseudomonas putida |  |
| Cu2+ | 0.01 mM | Pseudomonas putida | |
| Hg2+ | 0.01 mM | Pseudomonas putida | |
| p-mercuribenzoate | 1 mM, 85% inhibition | Pseudomonas putida | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.4 | - |
1-pyrroline-4-hydroxy-2-carboxylate | - |
Pseudomonas putida | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62000 | - |
ultracentrifugation | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-1-pyrroline-4-hydroxy-2-carboxylate + H2O | Pseudomonas putida | - |
2,5-dioxopentanoate + NH3 | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas putida |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.78 | - |
- |
Pseudomonas putida |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -15°C, fraction IV, at least one month | Pseudomonas putida |
| 25°C,fraction IV, at least three hours | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-1-pyrroline-4-hydroxy-2-carboxylate + H2O | - |
Pseudomonas putida | 2,5-dioxopentanoate + NH3 | - |
ir | |
| L-1-pyrroline-4-hydroxy-2-carboxylate + H2O | - |
Pseudomonas putida | 2,5-dioxopentanoate + NH3 | - |
ir | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Pseudomonas putida |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
40 | - |
Pseudomonas putida |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
fraction IV, 5 min, 10% loss in activity | Pseudomonas putida |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7.5 | - |
Pseudomonas putida |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | below pH 6 rapid decomposition of substrate | Pseudomonas putida |
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Release 2023.1 (January 2023)
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