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Literature summary for 3.5.4.2 extracted from

  • Matsui, H.; Shimaoka, M.; Kawasaki, H.; Takenaka, Y.; Kurahashi, O.
    Adenine deaminase activity of the yicP gene product of Escherichia coli (2001), Biosci. Biotechnol. Biochem., 65, 1112-1118.
    View publication on PubMed
Search for more literature for 3.5.4.2

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
Mn2+ required, activation at 1-5 mM, inhibitory above Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.8
-
 adenine pH 7.5, 37°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 11% of the activity with Mn2+ Escherichia coli
Fe2+ 48% of the activity with Mn2+ Escherichia coli
Mn2+ required, optimal at 1-5 mM, inhibitory above Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 2 * 60000, SDS-PAGE Escherichia coli
120000
-
 gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombonant protein Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.97
-
 pH 7.5, 37°C Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenine + H2O specific substrate Escherichia coli hypoxanthine + NH3
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 60000, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
 Escherichia coli